CTLA-4 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_005205. The protein contains a C-terminal DDK tag.
Homo sapiens CTLA4, mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for CTLA-4
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for CTLA-4 Overexpression Lysate (Native)
- CD152 antigen
- celiac disease 3
- cytotoxic T-lymphocyte antigen 4
- cytotoxic T-lymphocyte protein 4
- Cytotoxic T-lymphocyte-associated antigen 4
- cytotoxic T-lymphocyte-associated protein 4
- cytotoxic T-lymphocyte-associated serine esterase-4
- ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mk
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Applications: WB, IHC
Applications: ELISA, ICC/IF
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Applications: WB, AgAct
Species: Hu, Mu
Applications: Flow, Func, In vitro, In vivo, CyTOF-ready, Flow-CS
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
Applications: WB, IHC
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo, CyTOF-ready
Applications: WB, Flow, CyTOF-ready
Species: Hu, Ca, GP
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP
Publications for CTLA-4 Lysate (NBP2-07774) (0)
There are no publications for CTLA-4 Lysate (NBP2-07774).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for CTLA-4 Lysate (NBP2-07774) (0)
There are no reviews for CTLA-4 Lysate (NBP2-07774).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
FAQs for CTLA-4 Lysate (NBP2-07774) (0)
Additional CTLA-4 Products
Bioinformatics Tool for CTLA-4 Lysate (NBP2-07774)
Discover related pathways, diseases and genes to CTLA-4 Lysate (NBP2-07774). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on CTLA-4.
Thomson Reuters Predicts 2016 Nobel Prize Winners
Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro... Read full blog post.
CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated... Read full blog post.