CTGF/CCN2 Antibody - BSA Free

Western Blot: CTGF/CCN2 Antibody [NBP2-16026] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NBP2-16026] - Mouse placenta. CTGF antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: CTGF Antibody [NBP2-16026] - Sample (30 ug of whole cell lysate) A: HUVEC 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: CTGF Antibody [NBP2-16026] - Sample (50 ug of whole cell lysate) A: Rat bladder, 10% SDS PAGE gel, diluted at 1:500.

Western Blot: CTGF/CCN2 Antibody [NBP2-16026] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Pm, Sh
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CTGF/CCN2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human CTGF/CCN2. The exact sequence is proprietary.
• Localization: Secreted; extracellular space; extracellular matrix
• Predicted Species: Porcine (97%), Chimpanzee (99%), Bovine (97%), Sheep (99%), Chicken (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCN2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for CTGF/CCN2 Antibody - BSA Free

• CCN2
• CCN2IGFBP-8
• connective tissue growth factor
• CTGF
• CTGRP
• Fisp12
• HCS24
• Hypertrophic chondrocyte-specific protein 24
• IBP-8
• IGF-binding protein 8
• IGFBP-8
• IGFBP8CCN family member 2
• Insulin-like growth factor-binding protein 8
• MGC102839
• NOV2

Background

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CTGF/CCN2 Antibody (NBP2-16026)(1)

Publication using NBP2-16026

• Li S, Livingston MJ, Ma Z et al. Tubular cell senescence promotes maladaptive kidney repair and chronic kidney disease after cisplatin nephrotoxicity JCI insight 2023-03-14 [PMID: 36917180] (WB, Rat)

FAQs for CTGF/CCN2 Antibody (NBP2-16026). (Showing 1 - 1 of 1 FAQ).

1. We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
   • Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.

Bioinformatics

• Gene Symbol: CCN2
• Uniprot:
  • P29279()