CTGF/CCN2 Antibody

Western Blot: CTGF/CCN2 Antibody [NBP2-16025] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NBP2-16025] - Human gastric cancer, using CTGF antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: CTGF/CCN2 Antibody [NBP2-16025] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: CTGF/CCN2 Antibody [NBP2-16025] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Pm, Po, Ch, Pm, Sh
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CTGF/CCN2 Antibody Summary

• Description: Novus Biologicals Rabbit CTGF/CCN2 Antibody (NBP2-16025) is a polyclonal antibody validated for use in IHC and WB. Anti-CTGF/CCN2 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CTGF/CCN2. The exact sequence is proprietary.
• Localization: Secreted; extracellular space; extracellular matrix
• Predicted Species: Porcine (95%), Primate (99%), Chicken (94%), Sheep (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCN2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen Assay dependent
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for CTGF/CCN2 Antibody

• CCN2
• CCN2IGFBP-8
• connective tissue growth factor
• CTGF
• CTGRP
• Fisp12
• HCS24
• Hypertrophic chondrocyte-specific protein 24
• IBP-8
• IGF-binding protein 8
• IGFBP-8
• IGFBP8CCN family member 2
• Insulin-like growth factor-binding protein 8
• MGC102839
• NOV2

Background

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CTGF/CCN2 Antibody (NBP2-16025)(4)

Publications using NBP2-16025

• Livingston MJ, Shu S, Fan Y et al. Tubular cells produce FGF2 via autophagy after acute kidney injury leading to fibroblast activation and renal fibrosis Autophagy 2022-05-18 [PMID: 35491858] (WB, Mouse, Human)
• Ma Z, Hu X, Ding HF et al. Single-nucleus transcriptional profiling of chronic kidney disease after cisplatin nephrotoxicity The American journal of pathology 2022-01-26 [PMID: 35092726] (WB, Mouse)
• Choe JY, Son DS, Kim Y et al. L-type Calcium Channel Blocker Enhances Cellular Delivery and Gene Silencing Potency of Cell-Penetrating Asymmetric siRNAs Mol. Pharm. 2020-01-24 [PMID: 31976668]
• Livingston MJ, Ding HF, Huang S et al. Persistent activation of autophagy in kidney tubular cells promotes renal interstitial fibrosis during unilateral ureteral obstruction. Autophagy. 2016-04-28 [PMID: 27123926]

Review for CTGF/CCN2 Antibody (NBP2-16025) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/08/2019

Summary

• Application: Western Blot
• Sample Tested: 3 human pancreas cancer cell lines
• Species: Human
• Lot: 41794

FAQs for CTGF/CCN2 Antibody (NBP2-16025). (Showing 1 - 1 of 1 FAQs).

1. We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
   • Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.

Bioinformatics

• Gene Symbol: CCN2
• Uniprot:
  • P29279()