CSRP3 Antibody - BSA Free

Western Blot: CSRP3 Antibody [NBP1-32974] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CSRP3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: CSRP3 Antibody [NBP1-32974] - Paraformaldehyde-fixed HeLa, using CSRP3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.

Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-32974] - Mouse heart. CSRP3 stained by CSRP3 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-32974] - C2C12 xenograft. CSRP3 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-32974] - Mouse muscle. CSRP3 stained by CSRP3 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: CSRP3 Antibody [NBP1-32974] - Human heart (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CSRP3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CSRP3 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CSRP3. The exact sequence is proprietary.
• Localization: Nucleus, Cytoplasm, cytoskeleton
• Predicted Species: Porcine (98%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSRP3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for CSRP3 Antibody - BSA Free

• Cardiac LIM protein
• CLPMGC14488
• CMD1Mcardiac LIM domain protein
• CMH12
• CRP3cysteine and glycine-rich protein 3
• cysteine and glycine-rich protein 3 (cardiac LIM protein)
• Cysteine-rich protein 3
• LIM domain only 4
• LIM domain protein, cardiac
• LMO4
• MLPMGC61993
• Muscle LIM protein

Background

This gene encodes a member of the CSRP family of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this protein is found in a group of proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Mutations in this gene are thought to cause heritable forms of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) in humans. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CSRP3