CRM1 Antibody

Images

 
Immunoprecipitation: CRM1 Antibody [NB100-79811] - Detection of CRM1 (Exportin 1) by Immunoprecipitation. Samples: Whole cell lysate (1 mg/IP; 1/4 of reaction loaded/lane) from HeLa cells. Antibodies: Affinity purified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CRM1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 375 and 425 of human Chromosome Region Maintenance 1 (Exportin 1) using the numbering given in entry NP_003391.1 (GeneID 7514).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XPO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CRM1 Antibody

  • Chromosome region maintenance 1 protein homolog
  • DKFZp686B1823
  • emb
  • exp1
  • exportin 1 (CRM1 homolog, yeast)
  • exportin 1 (CRM1, yeast, homolog)
  • Exportin-1 (required for chromosome region maintenance)
  • exportin-1
  • yeast, homolog

Background

CRM1 mediates the nuclear export of cellular proteins (cargoes) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, CRM1 binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The direction of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. CRM1 also mediates the nuclear export of the Rev protein of HIV-1 and Rex protein of HTLV-1. It is involved in Rex dimerization, U3 snoRNA transport from Cajal bodies to nucleoli. CRM1 is also involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. CRM1 is also involved in the regulation NFAT and AP-1. Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in an approx. 25 nm channel used for larger, actively transported molecules. Active transport of molecules through the NPC is a signal-mediated process that is not well understood. Proteins to be exported from the nucleus to cytoplasm contain leucine-rich nuclear export signals (NESs). NESs are recognized by transport receptors belonging to the importin-beta family of proteins. It has been shown that NES export is mediated by CRM 1 (Chromosome Region Maintenance 1), an importin-beta family member that binds NESs, Ran:GTP, and Ran binding protein (RanBP). CRM 1 mediates the nuclear exportation of numerous proteins, including histone deacetylases (HDACs), viral proteins, immunophilins, and STAT 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB1259
Species: Hu
Applications: CyTOF-reported, ICC, ICFlow
NBP1-31776
Species: Hu, Mu
Applications: WB, ICC/IF
NBP1-30032
Species: Hu, Mu, Bv, Xp
Applications: WB, IHC, IHC-Fr
NBP1-79413
Species: Hu
Applications: WB
NB100-74480
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-52553
Species: Hu
Applications: WB, ELISA, Flow, CyTOF-ready
NBP2-56413
Species: Hu
Applications: WB, ICC/IF
H00001434-M08
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NB100-79814
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NB100-2244
Species: Hu
Applications: WB, ICC/IF, IHC-P, IP
NBP1-31649
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF1555
Species: Hu
Applications: WB
NB110-61646
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-31101
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89605
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-79811
Species: Hu
Applications: IP, WB (-)

Publications for CRM1 Antibody (NB100-79811) (0)

There are no publications for CRM1 Antibody (NB100-79811).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CRM1 Antibody (NB100-79811) (0)

There are no reviews for CRM1 Antibody (NB100-79811). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CRM1 Antibody (NB100-79811) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CRM1 Products

Bioinformatics Tool for CRM1 Antibody (NB100-79811)

Discover related pathways, diseases and genes to CRM1 Antibody (NB100-79811). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CRM1 Antibody (NB100-79811)

Discover more about diseases related to CRM1 Antibody (NB100-79811).
 

Pathways for CRM1 Antibody (NB100-79811)

View related products by pathway.

PTMs for CRM1 Antibody (NB100-79811)

Learn more about PTMs related to CRM1 Antibody (NB100-79811).
 

Research Areas for CRM1 Antibody (NB100-79811)

Find related products by research area.

Blogs on CRM1.

Survivin Acetylation: Affecting Apoptosis and Cancer
Survivin (BRIC5) is an inhibitor of apoptosis that also promotes cellular adaptation under stressful conditions and helps to regulate cell division. Recently, an antibody study by Dr. H Wang et al. at Brown University [PMID: 20826784] found that Survi...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CRM1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol XPO1
Entrez
Uniprot