CRM1 Antibody - BSA Free

Immunoprecipitation: CRM1 Antibody [NB100-79811] - Detection of CRM1 (Exportin 1) by Immunoprecipitation. Samples: Whole cell lysate (1 mg/IP; 1/4 of reaction loaded/lane) from HeLa cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody BL1956 (Cat. No. NB100-79811) used at 3 mcg/mg lysate for IP.

• Reactivity: Hu
• Applications: IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CRM1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CRM1 Antibody - BSA Free (NB100-79811) is a polyclonal antibody validated for use in WB and IP. Anti-CRM1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 375 and 425 of human Chromosome Region Maintenance 1 (Exportin 1) using the numbering given in entry NP_003391.1 (GeneID 7514).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XPO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CRM1 Antibody - BSA Free

• Chromosome region maintenance 1 protein homolog
• DKFZp686B1823
• emb
• exp1
• exportin 1 (CRM1 homolog, yeast)
• exportin 1 (CRM1, yeast, homolog)
• Exportin-1 (required for chromosome region maintenance)
• exportin-1
• yeast, homolog

Background

CRM1 mediates the nuclear export of cellular proteins (cargoes) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, CRM1 binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The direction of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. CRM1 also mediates the nuclear export of the Rev protein of HIV-1 and Rex protein of HTLV-1. It is involved in Rex dimerization, U3 snoRNA transport from Cajal bodies to nucleoli. CRM1 is also involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. CRM1 is also involved in the regulation NFAT and AP-1. Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in an approx. 25 nm channel used for larger, actively transported molecules. Active transport of molecules through the NPC is a signal-mediated process that is not well understood. Proteins to be exported from the nucleus to cytoplasm contain leucine-rich nuclear export signals (NESs). NESs are recognized by transport receptors belonging to the importin-beta family of proteins. It has been shown that NES export is mediated by CRM 1 (Chromosome Region Maintenance 1), an importin-beta family member that binds NESs, Ran:GTP, and Ran binding protein (RanBP). CRM 1 mediates the nuclear exportation of numerous proteins, including histone deacetylases (HDACs), viral proteins, immunophilins, and STAT 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRM1 Antibody (NB100-79811)(1)

Publication using NB100-79811

• Brinton LT, Sher S, Williams K et al. Cotargeting of XPO1 Enhances the Antileukemic Activity of Midostaurin and Gilteritinib in Acute Myeloid Leukemia Cancers (Basel) 2020-06-14 [PMID: 32545904] (WB, Human)

Bioinformatics

• Gene Symbol: XPO1
• Entrez:
  • 7514(Human)
• Uniprot:
  • NP_003391.1(Human)