Genetic Strategies: Western Blot: CRBN Antibody [NBP1-91810] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: ...read more
Immunocytochemistry/ Immunofluorescence: CRBN Antibody [NBP1-91810] - Staining of human cell line U-251 MG shows localization to nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CRBN Antibody [NBP1-91810] - Staining of human skeletal muscle shows only weak cytoplasmic positivity in striated muscle fibers.
Western Blot: CRBN Antibody [NBP1-91810] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: CRBN Antibody [NBP1-91810] - Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: CRBN Antibody [NBP1-91810] - Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: CRBN Antibody [NBP1-91810] - Staining of human colon shows moderate cytoplasmic and nuclear positivity in peripheral ganglion.
Simple Western: CRBN Antibody [NBP1-91810] - Simple Western lane view shows a specific band for CRBN in 0.2 mg/ml of RT4 (left), U-251MG (right) lysate. This experiment was performed under reducing conditions using ...read more
Simple Western: CRBN Antibody [NBP1-91810] - Electropherogram image(s) of corresponding Simple Western lane view. CRBN antibody was used at 1:60 dilution on RT4 and U-251MG lysate(s).
Genetic Strategies: Single Cell Western: CRBN Antibody [NBP1-91810] - CRBN expression after Downregulation by siRNA, dilution 1:500. Single Cell Western Image submitted by verified customer review.
Knockdown Validated: CRBN Antibody [NBP1-91810] - Validation of SALL4 asa bona fide IMiD-dependent CRL4CRBN substrate. Parental Kelly cells or two independent pools of CRBN -/- Kelly cells were treated with increasing ...read more
Hu, Mu, RtSpecies Glossary
WB, Simple Western, ICC/IF, IHC, IHC-P, KD, KO, Single-Cell Western
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Knockout validation (PMID: 31371704).
FAQs for CRBN Antibody (NBP1-91810). (Showing 1 - 1 of 1 FAQs).
For the picture on your website, you run four cell lines. The lane 2 and 3 all have two bands. Which is the CRBN?
Human CRBN has two known isoforms, both of which are approximately the same molecular weight, 50kDa. Given this information, it would be extremely difficult to distinguish the two in a WB. In our testing of the two cell lines, we did get multiple bands very close to each other, while in other lines or tissue lysates, we get one clean band. Unfortunately, we have not yet fully investigated the multiple banding pattern shown in the image. The antibody is polyclonal, so we typically tend to see some non-specific banding that can be cleaned up with further protocol optimization.
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