SH3BP2 Antibody (1E9)

Western Blot: SH3BP2 Antibody (1E9) [H00006452-M01] - SH3BP2 monoclonal antibody (M01), clone 1E9 Analysis of SH3BP2 expression in A-431.

Immunocytochemistry/Immunofluorescence: SH3BP2 Antibody (1E9) [H00006452-M01] - Analysis of monoclonal antibody to SH3BP2 on A-431 cell. Antibody concentration 20 ug/ml.

Sandwich ELISA: SH3BP2 Antibody (1E9) [H00006452-M01] - Detection limit for recombinant GST tagged SH3BP2 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 1E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SH3BP2 Antibody (1E9) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SH3BP2 (AAH22996, 452 a.a. ~ 561 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLPNSVFVNTTESCEVERLFKATSPRGEPQDGLYCIRNSSTKSGKVLVVWDETSNKVRNYRIFEKDSKFYLEGEVLFVSVGSMVEHYHTHVLPSHQSLLLRHPYGYTGPR
• Specificity: SH3BP2 - SH3-domain binding protein 2
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SH3BP2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SH3BP2 Antibody (1E9)

• Abl-SH3 binding protein 2
• Cherubism
• CRBM
• CRPM
• FLJ42079
• FLJ54978,3BP-2,3BP2
• RES4-23
• SH3 domain-binding protein 2
• SH3-domain binding protein 2
• TNFAIP3 interacting protein 2

Background

The protein encoded by the SH3BP2 gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SH3BP2 Antibody (H00006452-M01)(3)

Publications using H00006452-M01

• Kyoko K, Tomoyuki M, Masanori I et al. SH3BP2 Deficiency Ameliorates Murine Systemic Lupus Erythematosus. Int J Mol Sci. 2021-04-17 [PMID: 33920631]
• Kittaka M, Yoshimoto T, Schlosser C et al. Alveolar bone protection by targeting the SH3BP2-SYK axis in osteoclasts. J Bone Miner Res. 2019-10-24 [PMID: 31613396]
• Matsumoto Y, La Rose J, Kent OA et al. Reciprocal stabilization of ABL and TAZ regulates osteoblastogenesis through transcription factor RUNX2. J Clin Invest 2016-12-01 [PMID: 27797343]

Bioinformatics

• Gene Symbol: SH3BP2
• Entrez:
  • 6452(Human)
• OMIM:
  • 118(Human)
• Uniprot:
  • AAH22996(Human)