beta-Arrestin 2 Antibody - BSA Free

Western Blot: beta-Arrestin 2 Antibody [NBP2-24569] - Analysis of ARRB2 in HUVEC cell lysate in the 1) absence and 2) presence of immunizing peptide using ARRB2 antibody at 0.25 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used.

Immunohistochemistry-Paraffin: beta-Arrestin 2 Antibody [NBP2-24569] - Human thyroid tissue stained with ARRB2 antibody at 5 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Simple Western: beta-Arrestin 2 Antibody [NBP2-24569] - Simple Western lane view shows a specific band for beta Arrestin in 0.5 mg/mL of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: beta-Arrestin 2 Antibody [NBP2-24569] - Beta-arrestin antibody detects 2 peaks at 32 and 45 kDa using Simple Western. Traditional size of beta-arrestin 2 is ~55 kDa. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Eq, Pm, Xp, Ze
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

beta-Arrestin 2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit beta-Arrestin 2 Antibody - BSA Free (NBP2-24569) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-beta-Arrestin 2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A peptide sequence corresponding to an area between amino acids 1 and 50 of human ARRB2 was used as the immunogen for the antibody, Gen Bank no. gb:ABG47460.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARRB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Simple Western 1:100
  • Western Blot 0.25 - 0.5 ug/mL
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Jurkat lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100, apparent MW was 54 kDa

Reactivity Notes

Chimpanzee, platypus, opssoum, boar (100%), Tetradon (94%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for beta-Arrestin 2 Antibody - BSA Free

• ARB2
• ARR2
• ARRB2
• arrestin 3
• Arrestin beta-2
• arrestin, beta 2
• BARR2
• betaArrestin 2
• beta-Arrestin 2
• beta-arrestin-2
• DKFZp686L0365

Background

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Arrestin 2 Antibody (NBP2-24569)(2)

Publications using NBP2-24569

• Wang W, Li Y, Qu X et al. MiR-365-3p is Involved in IL17-Mediated Asthma in Mouse Model Front Immunol 2022-08-12 [PMID: 35958620]
• Nogueras-Ortiz C, Roman-Vendrell C, Mateo-Semidey GE et al. Retromer stops beta-arrestin 1 mediated signaling from internalized cannabinoid 2 receptors. Mol. Biol. Cell. 2017-09-27 [PMID: 28954865]

Review for beta-Arrestin 2 Antibody (NBP2-24569) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Simple Western Mouse 11/16/2018

Summary

• Application: Simple Western
• Sample Tested: Mouse skeletal muscle
• Species: Mouse
• Lot: 11203480B

Comments

• Comments: antibody used at 1:50 dilution
  0.5 mg/ml protein concentration of the sample
  GAPDH, 1:150 dilution, (14C10, HRP Conjugate, #3683 CST) used as loading control (~39 kDa)

Bioinformatics

• Gene Symbol: ARRB2