RUNX3/CBFA3 Antibody (527327) [Unconjugated]


Human PBMC unstimulated (light orange filled histogram) or treated with 50ng/mL PMA (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse RUNX3/CBFA3 Monoclonal Antibody (Catalog # MAB3765) or isotype more
RUNX3/CBFA3 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human/Mouse RUNX3/CBFA3 Monoclonal Antibody (Catalog # MAB3765) at 10 µg/mL for 3 hours at room temperature. more
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Daudi human Burkitt's lymphoma cell line, and DA3 mouse myeloma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/mL. A specific band was detected for RUNX3/CBFA3 at approximately 54 more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, CyTOF-ready, Flow, ICC/IF

Order Details

RUNX3/CBFA3 Antibody (527327) [Unconjugated] Summary

E. coli-derived recombinant human RUNX3/CBFA3
Accession # Q13761
Detects human and mouse RUNX3/CBFA3 in Western blots.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publications using
MAB3765 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RUNX3/CBFA3 Antibody (527327) [Unconjugated]

  • Acute myeloid leukemia 2 protein
  • AML2
  • AML2SL3/AKV core-binding factor alpha C subunit
  • CBFA3
  • CBFA3MGC16070
  • CBF-alpha-3
  • PEA2 alpha C
  • PEA2-alpha C
  • PEBP2 alpha C
  • PEBP2A3
  • PEBP2A3FLJ34510
  • PEBP2-alpha C
  • runt domain, alpha subunit 3
  • runt-related transcription factor 3
  • RUNX3
  • SL3-3 enhancer factor 1 alpha C subunit
  • transcription factor AML2


RUNX3, also called CBFA3, AML-2 or PEBP2-alpha C, is a member of the Runt domain family of nuclear transcriptional regulators. All of the RUNX proteins form dimers with CBF-beta . The runt domain (aa 54-186) is required for DNA binding, while a pro/ser/thr-rich region (aa 191-415) transcriptionally activates target genes. Isoform 2 has an alternate 19 aa in place of the N-terminal 5 aa of isoform 1. The 415 aa Human RUNX3 shares 91% aa identity with mouse or rat RUNX3. RUNX3 is necessary for growth control of gastric epithelium, neurogenesis of dorsal root ganglia, and T cell differentiation. RUNX3 expression is frequently mutated in tumors and appears to be silenced by methylation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RUNX3/CBFA3 Antibody (MAB3765)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow Cytometry, ICC, Western Blot.

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Showing Publications 1 - 4 of 4.
Publications using MAB3765 Applications Species
F Facciotti, P Larghi, R Bosotti, C Vasco, N Gagliani, C Cordiglier, S Mazzara, V Ranzani, E Rottoli, S Curti, A Penatti, B Karnani, Y Kobayashi, M Crosti, M Bombaci, JP van Hambur, G Rossetti, R Gualtierot, M Gerosa, S Gatti, S Torretta, L Pignataro, SW Tas, S Abrignani, M Pagani, F Grassi, PL Meroni, RA Flavell, J Geginat Evidence for a pathogenic role of extrafollicular, IL-10-producing CCR6+B helper T cells in systemic lupus erythematosus Proc. Natl. Acad. Sci. U.S.A., 2020-03-17;117(13):7305-7316. 2020-03-17 [PMID: 32184325] (Flow Cytometry, Human) Flow Cytometry Human
P Balogh, ER Adelman, JV Pluvinage, BJ Capaldo, KC Freeman, S Singh, KE Elagib, Y Nakamura, R Kurita, G Sashida, ER Zunder, H Li, AA Gru, EA Price, SL Schrier, IL Weissman, ME Figueroa, WW Pang, AN Goldfarb RUNX3 levels in human hematopoietic progenitors are regulated by aging and dictate erythroid-myeloid balance Haematologica, 2019-06-06;0(0):. 2019-06-06 [PMID: 31171641] (ICC, Human) ICC Human
Chopin M, Seillet C, Chevrier S, Wu L, Wang H, Morse H, Belz G, Nutt S Langerhans cells are generated by two distinct PU.1-dependent transcriptional networks. J Exp Med, 2013-11-18;210(13):2967-80. 2013-11-18 [PMID: 24249112] (Flow Cytometry, Mouse) Flow Cytometry Mouse
NOTCH1 promotes T cell leukemia-initiating activity by RUNX-mediated regulation of PKC-theta and reactive oxygen species. Nat Med, 2012-10-21;18(11):1693-8. 2012-10-21 [PMID: 23086478] (Western Blot, Mouse) Western Blot Mouse

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Gene Symbol RUNX3