Neurotrypsin Antibody

Images

 
Western Blot: Neurotrypsin Antibody [NBP1-76784] - Analysis in in 3T3 cell lysate with antibody at (A) 0.25 and (B) 0.5 ug/mL.
Immunocytochemistry/ Immunofluorescence: Neurotrypsin Antibody [NBP1-76784] - Immunofluorescence of Neurotrypsin in Human Brain cells with Neurotrypsin antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: Neurotrypsin Antibody [NBP1-76784] - Human brain tissue with Neurotrypsin antibody at 2.5 ug/ml.
Western Blot: Neurotrypsin Antibody [NBP1-76784] - SK-N-SH cell lysate with neurotrypsin antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Neurotrypsin Antibody Summary

Immunogen
Antibody was raised against a 23 amino acid synthetic peptide from near the amino terminus of human neurotrypsin. The immunogen is located within amino acids 110 - 160 of Neurotrypsin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRSS12
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Neurotrypsin Peptide (NBP1-76784PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Neurotrypsin Antibody

  • brain-specific serine protease 3
  • BSSP3
  • BSSP-3
  • EC 3.4.21
  • Leydin
  • MGC12722
  • Motopsin
  • MRT1EC 3.4.21.-
  • neurotrypsin
  • protease, serine, 12 (neurotrypsin, motopsin)
  • Serine protease 12

Background

Neurotrypsin is a central nervous system-expressed serine protease whose truncation or absence causes nonsyndromic mental retardation. It is most prominently expressed in structures that are involved in the processing and storage of learned behavior and memory, such as the cerebral cortex, the hippocampus, and amygdala. Evidence suggests that neurotrypsin has multiple functions, including axonal outgrowth, maintaining neuronal plasticity, and arranging the perineuronal environment, partly in coordination with other proteases including tissue plasminogen activator. At least two isoforms of neurotrypsin are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRSS12
Uniprot