CC2D1A Antibody - BSA Free

Western Blot: CC2D1A Antibody [NB500-255] - Detection of Human TOUF/FLJ20241 on HeLa whole cell lysate using NB500-255.

Immunohistochemistry-Paraffin: CC2D1A Antibody [NB500-255] - Human colon carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).

Western Blot: CC2D1A Antibody [NB500-255] - Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CC2D1A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CC2D1A Antibody - BSA Free (NB500-255) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-CC2D1A Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Coiled-coil and C2 Domain Containing 1A using the numbering given in TrEMBL entry Q6P1N0 (GeneID 54862).
• Specificity: NB500-255 is specific for human TOUF/FLJ-20241 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CC2D1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 1 -4 ug/mg lysate
  • Western Blot 1:1000 - 1:10000
• Application Notes: This antibody can be used in Western Blot, Immunofluorescence, and Immunoprecipitation. Recommended Starting Dilutions*: WB: 1:1,000-1:10,000 IP: 1-4 ug/mg lysate IF: 1/100 *Optimal dilutions should be determined by the end user.
• Control:

  CC2D1A Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CC2D1A Antibody - BSA Free

• Akt kinase-interacting protein 1
• coiled-coil and C2 domain containing 1A
• coiled-coil and C2 domain-containing protein 1A
• Five repressor element under dual repression-binding protein 1
• FLJ20241
• FLJ41160
• FRE under dual repression-binding protein 1
• FREUD-1
• mental retardation, nonsyndromic, autosomal recessive, 3
• MRT3Freud-1/Aki1
• Putative NF-kappa-B-activating protein 023N
• putative NFkB activating protein

Background

TOUF, or Cc2d1a, is a signal transducer that participates in the positive regulation of the I-kappaB kinase/NF-kappaB cascade. Cc2d1a has been implicated as playing an important role in human cognitive development, with a particular relation to Nonsyndromic Mental Retardation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CC2D1A Antibody (NB500-255)(3)

Publications using NB500-255

• Usami Y, Popov S, Weiss ER et al. Regulation of CHMP4/ESCRT-III function in human immunodeficiency virus type 1 budding by CC2D1A. J Virol 2012-04-01 [PMID: 22258254]
• Rogaeva A, Ou XM, Jafar-Nejad H et al. Differential repression by freud-1/CC2D1A at a polymorphic site in the dopamine-D2 receptor gene. J Biol Chem 2007-07-01 [PMID: 17535813]
• Basel-Vanagaite L, Attia R, Yahav M et al. The CC2D1A, a member of a new gene family with C2 domains, is involved in autosomal recessive non-syndromic mental retardation. J Med Genet 2006-03-01 [PMID: 16033914]

Bioinformatics

• Gene Symbol: CC2D1A
• Entrez:
  • 54862(Human)
• Uniprot:
  • Q6P1N0(Human)