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CC2D1A Antibody

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Western Blot: CC2D1A Antibody [NB500-255] - Detection of Human TOUF/FLJ20241 on HeLa whole cell lysate using NB500-255.
Western Blot: CC2D1A Antibody [NB500-255] - Detection of Human TOUF/FLJ20241 on HeLa whole cell lysate using NB500-255.
Immunohistochemistry-Paraffin: CC2D1A Antibody [NB500-255] - Human colon carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
Western Blot: CC2D1A Antibody [NB500-255] - Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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CC2D1A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Coiled-coil and C2 Domain Containing 1A using the numbering given in TrEMBL entry Q6P1N0 (GeneID 54862).
Specificity
NB500-255 is specific for human TOUF/FLJ-20241 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CC2D1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 1 -4 ug/mg lysate
  • Western Blot 1:1000 - 1:10000
Application Notes
This antibody can be used in Western Blot, Immunofluorescence, and Immunoprecipitation. Recommended Starting Dilutions*: WB: 1:1,000-1:10,000 IP: 1-4 ug/mg lysate IF: 1/100 *Optimal dilutions should be determined by the end user.
Control
CC2D1A Overexpression Lysate
Publications
Read Publications using NB500-255.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CC2D1A Antibody

  • Akt kinase-interacting protein 1
  • coiled-coil and C2 domain containing 1A
  • coiled-coil and C2 domain-containing protein 1A
  • Five repressor element under dual repression-binding protein 1
  • FLJ20241
  • FLJ41160
  • FRE under dual repression-binding protein 1
  • FREUD-1
  • mental retardation, nonsyndromic, autosomal recessive, 3
  • MRT3Freud-1/Aki1
  • Putative NF-kappa-B-activating protein 023N
  • putative NFkB activating protein

Background

TOUF, or Cc2d1a, is a signal transducer that participates in the positive regulation of the I-kappaB kinase/NF-kappaB cascade. Cc2d1a has been implicated as playing an important role in human cognitive development, with a particular relation to Nonsyndromic Mental Retardation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: Neurotrypsin Antibody [NBP1-76784] - Human brain tissue with Neurotrypsin antibody at 2.5 ug/ml.
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Immunohistochemistry: TLR4 Antibody (76B357.1) [NB100-56566] - Pericryptal Myofibroblasts are Responsible for Increased TLR4 Expression in a Subset of CRCs. Double-stained immunofluorescence for TLR4 (green) and vimentin (red) in normal (I), adenoma (II), and colon adenocarcinoma (III) (10x). In the stromal compartment of CRCs, immunofluorescent staining for TLR4 localized to the pericryptal myofibroblasts in a subset of samples. Image collected and cropped by CiteAb from the following publication (http://www.jeccr.com/content/33/1/45), licensed under a CC-BY licence.
(5 Reviews)
(114 Publications)
Species: Bv, Hu, Ma, Mu, Po, Rt
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(40C1285.6) - BSA Fre
NBP2-24875
Flow Cytometry: TLR3 Antibody (40C1285.6) [NBP2-24875] - Expression of TLR3 protein on epithelial cells. HNEC (A), Detroit-562 (B) and FaDu (C) were stained intracellularly with FITC-Abs against TLR3 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0098239), licensed under a CC-BY licence.
Western Blot: TLR3 Antibody (40C1285.6) [NBP2-24875] - All the cell lines displayed a strong expression of TLR3 protein. Cell lysates were electrophoresed and blotted to PVDF membrane, which was probed with TLR3-specific antibody. To confirm the immunoreactivity of the antibody, different positive controls were included. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY licence.
Flow Cytometry: TLR3 Antibody (40C1285.6) [NBP2-24875] - Expression of TLR3 in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry. HCMLs were stained using an intracellular staining protocol with TLR3 antibodies followed by relevant secondary fluorescent-conjugated antibodies. Filled histograms (purple) represent the isotype controls and the open histograms (red) indicate TLR3. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY licence.
(82 Publications)
Species: Ca, Hu, Mu
Applications: BA, B/N, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
sFRP-3/FRZB
7584-SF/CF
sFRP-3/FRZB
(1 Publication)
Species: Hu
Applications: BA

Publications for CC2D1A Antibody (NB500-255)(3)


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Control Lysate(s)

CC2D1A Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CC2D1A
Entrez
Uniprot