Western Blot: COX4 Antibody [NB110-39115] - analysis of COX4 in porcine whole skeletal muscle lysate using anti-COX4 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: COX4 Antibody [NB110-39115] - IF confocal analysis of HeLa cells using COX4 antibody (NB110-39115, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary ...read more
Immunohistochemistry: COX4 Antibody [NB110-39115] - IHC analysis of COX4 in human breast cancer using DAB with hematoxylin counterstain.
Western Blot: COX4 Antibody [NB110-39115] - WB analysis of COX4 in the following cell lysates: 1. HeLa, 2. Ntera, 3. A431, 4. HepG2, 5. MCF7 and 6. 3T3.
Western Blot: COX4 Antibody [NB110-39115] - Detection of COX41 using NB110-39115. Lane 1: HEK 293 with empty vector. Lane 2: HEK 293 with flag-tagged human COX4. Lane 3: HEK 293 with flag-tagged human COX42. Photo ...read more
Immunocytochemistry/ Immunofluorescence: COX4 Antibody [NB110-39115] - COX4 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Simple Western: COX4 Antibody [NB110-39115] - Simple Western lane view shows a specific band for COX4 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Immunogen affinity purified
Western Blot 1:2000
Simple Western 1:25
This COX IV isoform 1 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen ~19.5 kDa representing COX IV. In ICC/IF, mitochondrion staining was observed in HeLa cells. In IHC-P, staining is observed in the cytoplasm and mitochondria of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Higher dilutions may be needed for mitochondrial membrane enriched preparations.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read 3 Reviews rated 4.3 using NB110-39115 in the following applications:
Human, mouse, bovine, primate and rat. Immunogen sequence has 76% homology to Zebrafish.
Alternate Names for COX4 Antibody
Cytochrome c oxidase polypeptide IV
cytochrome c oxidase subunit 4 isoform 1, mitochondrial
cytochrome c oxidase subunit IV isoform 1cytochrome c oxidase subunit IV
Cytochrome C Oxidase (COX), which is located in the inner mitochondrial membrane, is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced Cytochrome C and donates them to molecular oxygen, which is then reduced to water.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
IF confocal analysis of HeLa cells using COX IV antibody (NB110-39115, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).