COX4 Antibody

Images

 
Western Blot: COX4 Antibody [NB110-39115] - WB detection of COX4-1 isoform using COX4 antibody NB110-39115. Lane 1: HEK 293 with empty vector, Lane 2: HEK 293 with flag-tagged human COX4-1, Lane 3: HEK 293 with ...read more
Immunocytochemistry/ Immunofluorescence: COX4 Antibody [NB110-39115] - IF confocal analysis of HeLa cells using COX4 antibody (NB110-39115, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary ...read more
Immunohistochemistry: COX4 Antibody [NB110-39115] - IHC analysis of COX4 in human breast cancer using DAB with hematoxylin counterstain.
Western Blot: COX4 Antibody [NB110-39115] - WB analysis of COX4 in the following cell lysates: 1. HeLa, 2. Ntera, 3. A431, 4. HepG2, 5. MCF7 and 6. 3T3.
Western Blot: COX4 Antibody [NB110-39115] - analysis of COX4 in porcine whole skeletal muscle lysate using anti-COX4 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: COX4 Antibody [NB110-39115] - COX4 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Simple Western: COX4 Antibody [NB110-39115] - Simple Western lane view shows a specific band for COX4 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Immunofluorescence: COX4 Antibody [NB110-39115] - Immunofluorescence staining analysis of methanol fixed drosophila embryo using 1:200 dilution of COX4 antibody. The signal was developed using AF488 conjugated Donkey ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Dr, I, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

COX4 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of human COX IV isoform 1 (within residues 1-100). [Swiss-Prot# P13073]
Localization
Mitochondrion, mito inner membrane
Marker
Mitochondria Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COX4I1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Simple Western 1:25
  • Immunocytochemistry/Immunofluorescence 1:40
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This COX IV isoform 1 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen ~19.5 kDa representing COX IV. In ICC/IF, mitochondrion staining was observed in HeLa cells. In IHC-P, staining is observed in the cytoplasm and mitochondria of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Higher dilutions may be needed for mitochondrial membrane enriched preparations.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
293 Lysate (NB800-PC6)
HeLa Lysate (NB800-PC1)
COX4 Lysate (NBL1-09409)
Reviewed Applications
Read 4 Reviews rated 4.5
using
NB110-39115 in the following applications:

Publications
Read Publications using
NB110-39115 in the following applications:

  • WB
    2 publications

Reactivity Notes

Human, mouse, bovine, primate and rat. Immunogen sequence has 76% homology to Zebrafish.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for COX4 Antibody

  • COX IV-1
  • COX4
  • COX4-1
  • COX4COXIV
  • COX4I2
  • Cytochrome c oxidase polypeptide IV
  • cytochrome c oxidase subunit 4 isoform 1, mitochondrial
  • cytochrome c oxidase subunit IV isoform 1cytochrome c oxidase subunit IV
  • MGC72016

Background

Cytochrome C Oxidase (COX), which is located in the inner mitochondrial membrane, is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced Cytochrome C and donates them to molecular oxygen, which is then reduced to water.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-39115
Species: Hu, Mu, Rt, Po, Bv, Dr, I, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for COX4 Antibody (NB110-39115)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
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Reviews for COX4 Antibody (NB110-39115) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Drosophila, Other.
We have 4 reviews tested in 2 application: WB, ICC.

Reviews using NB110-39115:
Filter by Applications
WB
(2)
ICC
(2)
All Applications
Filter by Species
Human
(1)
Drosophila
(1)
Other
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot COX4 NB110-39115
Enlarge
reviewed by:
ilaria drago
WB Drosophila 06/26/2017
View

Summary

ApplicationWestern Blot
Sample Tested 20 ug whole body lysate
SpeciesDrosophila

Comments

Comments20 ug whole body lysate run in a 12% gel.
Antibody used 1:4000
Immunocytochemistry COX4 NB110-39115
Enlarge
reviewed by:
Anonymous
ICC Other 10/29/2015
View

Summary

ApplicationImmunocytochemistry
SpeciesOther
FileView PDF
Western Blot COX4 NB110-39115
Enlarge
reviewed by:
Anonymous
WB Other 04/23/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate
SpeciesOther
LotB-1

Blocking

Blocking Details3% Casein in TBS with 0.1% Tween-20 for 1 hr

Primary Anitbody

Dilution Ratio1/1000 at 4C for 20 hr

Secondary Antibody

Secondary DescriptionDonkey anti-Rabbit IRDye 800CW - Licor Biosciences
Secondary Manufacturer Cat#926-32213
Secondary Concentration1/2500

Details

Detection NotesLicor Odyssey
Immunocytochemistry COX4 NB110-39115
Enlarge
reviewed by:
Anony- mous
ICC Human 06/16/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF confocal analysis of HeLa cells using COX IV antibody (NB110-39115, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for COX4 Antibody (NB110-39115) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-39115AF405
Alexa Fluor 488 Labeled NB110-39115AF488
Alexa Fluor 647 Labeled NB110-39115AF647
Alexa Fluor 700 Labeled NB110-39115AF700
Biotin Labeled NB110-39115B
DyLight 405 Labeled NB110-39115V
DyLight 488 Labeled NB110-39115G
DyLight 550 Labeled NB110-39115R
DyLight 650 Labeled NB110-39115C
FITC Labeled NB110-39115F
HRP Labeled NB110-39115H

Additional COX4 Products

Bioinformatics Tool for COX4 Antibody (NB110-39115)

Discover related pathways, diseases and genes to COX4 Antibody (NB110-39115). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for COX4 Antibody (NB110-39115)

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Pathways for COX4 Antibody (NB110-39115)

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Research Areas for COX4 Antibody (NB110-39115)

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Blogs on COX4.

The Importance of the COX IV Antibody to Apoptosis Research
COX IV isoform 1 is a nuclear-encoded polypeptide chain of the Cytochrome C Oxidase enzyme, located on the mitochondrial inner membrane. Owing to its widespread distribution in human and mammalian tissues, COX IV antibodies are widely used as loading...  Read full blog post.

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Recent Reviews

4.5
5
2
4
2
3
0
2
0
1
0

ilaria drago
06/26/2017
Application: WB
Species: Drosophila

Anonymous
10/29/2015
Application: ICC
Species: Other

Anonymous
04/23/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol COX4I1
Entrez