COX4 Antibody [HRP]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Dr, I, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

COX4 Antibody [HRP] Summary

Immunogen
A synthetic peptide made to an internal region of human COX IV isoform 1 (within residues 1-100). [Swiss-Prot# P13073]
Localization
Mitochondrion, mito inner membrane
Marker
Mitochondria Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COX4I1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This COX IV isoform 1 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen ~19.5 kDa representing COX IV. In ICC/IF, mitochondrion staining was observed in HeLa cells. In IHC-P, staining is observed in the cytoplasm and mitochondria of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Higher dilutions may be needed for mitochondrial membrane enriched preparations.
Readout System

Reactivity Notes

Human, mouse, bovine, primate and rat. Immunogen sequence has 76% homology to Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for COX4 Antibody [HRP]

  • COX IV-1
  • COX4
  • COX4-1
  • COX4COXIV
  • COX4I2
  • Cytochrome c oxidase polypeptide IV
  • cytochrome c oxidase subunit 4 isoform 1, mitochondrial
  • cytochrome c oxidase subunit IV isoform 1cytochrome c oxidase subunit IV
  • MGC72016

Background

Cytochrome C Oxidase (COX), which is located in the inner mitochondrial membrane, is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced Cytochrome C and donates them to molecular oxygen, which is then reduced to water.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-39115H
Species: Hu, Mu, Rt, Po, Bv, Dr, I, Pm
Applications: WB, IHC, IHC-P

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for COX4 Antibody (NB110-39115H) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB110-39115B
DyLight 488 Labeled NB110-39115G
DyLight 550 Labeled NB110-39115R
DyLight 650 Labeled NB110-39115C

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Bioinformatics Tool for COX4 Antibody (NB110-39115H)

Discover related pathways, diseases and genes to COX4 Antibody (NB110-39115H). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for COX4 Antibody (NB110-39115H)

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Pathways for COX4 Antibody (NB110-39115H)

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PTMs for COX4 Antibody (NB110-39115H)

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Research Areas for COX4 Antibody (NB110-39115H)

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Blogs on COX4.

The Importance of the COX IV Antibody to Apoptosis Research
COX IV isoform 1 is a nuclear-encoded polypeptide chain of the Cytochrome C Oxidase enzyme, located on the mitochondrial inner membrane. Owing to its widespread distribution in human and mammalian tissues, COX IV antibodies are widely used as loading...  Read full blog post.

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Bioinformatics

Gene Symbol COX4I1
Entrez