COX4 Antibody [Alexa Fluor® 405]

• Reactivity: Hu, Mu, Rt, Po, Bv, Dr, I, Ma-Op, Pm
• Applications: WB, ICC/IF, IHC, IHC-P, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 405

COX4 Antibody [Alexa Fluor® 405] Summary

• Immunogen: A synthetic peptide made to an internal region of human COX IV isoform 1 (within residues 1-100). [Swiss-Prot# P13073]
• Localization: Mitochondrion, mito inner membrane
• Marker: Mitochondria Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COX4I1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
• Application Notes: This COX IV isoform 1 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen ~19.5 kDa representing COX IV. In ICC/IF, mitochondrion staining was observed in HeLa cells. In IHC-P, staining is observed in the cytoplasm and mitochondria of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Higher dilutions may be needed for mitochondrial membrane enriched preparations.

Reactivity Notes

Opossum reactivity reported in scientific literature (PMID: 28720662).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for COX4 Antibody [Alexa Fluor® 405]

• COX IV-1
• COX4
• COX4-1
• COX4COXIV
• COX4I2
• Cytochrome c oxidase polypeptide IV
• cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• cytochrome c oxidase subunit IV isoform 1cytochrome c oxidase subunit IV
• MGC72016

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COX4I1