COX4 Antibody [Alexa Fluor® 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Dr, I, Ma-Op, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 405

COX4 Antibody [Alexa Fluor® 405] Summary

Immunogen
A synthetic peptide made to an internal region of human COX IV isoform 1 (within residues 1-100). [Swiss-Prot# P13073]
Localization
Mitochondrion, mito inner membrane
Marker
Mitochondria Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COX4I1
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
Application Notes
This COX IV isoform 1 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen ~19.5 kDa representing COX IV. In ICC/IF, mitochondrion staining was observed in HeLa cells. In IHC-P, staining is observed in the cytoplasm and mitochondria of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Higher dilutions may be needed for mitochondrial membrane enriched preparations.

Reactivity Notes

Opossum reactivity reported in scientific literature (PMID: 28720662).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for COX4 Antibody [Alexa Fluor® 405]

  • COX IV-1
  • COX4
  • COX4-1
  • COX4COXIV
  • COX4I2
  • Cytochrome c oxidase polypeptide IV
  • cytochrome c oxidase subunit 4 isoform 1, mitochondrial
  • cytochrome c oxidase subunit IV isoform 1cytochrome c oxidase subunit IV
  • MGC72016

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-39115AF405
Species: Hu, Mu, Rt, Po, Bv, Dr, I, Ma-Op, Pm
Applications: WB, ICC/IF, IHC, IHC-P, KD

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for COX4 Antibody (NB110-39115AF405) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-39115AF350
Alexa Fluor 405 NB110-39115AF405
Alexa Fluor 488 NB110-39115AF488
Alexa Fluor 532 NB110-39115AF532
Alexa Fluor 594 NB110-39115AF594
Alexa Fluor 647 NB110-39115AF647
Alexa Fluor 700 NB110-39115AF700
Alexa Fluor 750 NB110-39115AF750
Biotin NB110-39115B
DyLight 350 NB110-39115UV
DyLight 405 NB110-39115V
DyLight 488 NB110-39115G
DyLight 550 NB110-39115R
DyLight 594 NB110-39115DL594
DyLight 650 NB110-39115C
DyLight 680 NB110-39115FR
DyLight 755 NB110-39115IR
FITC NB110-39115F
HRP NB110-39115H
Janelia Fluor 549 NB110-39115JF549
Janelia Fluor 646 NB110-39115JF646

Additional COX4 Products

Array NB110-39115AF405

Bioinformatics Tool for COX4 Antibody (NB110-39115AF405)

Discover related pathways, diseases and genes to COX4 Antibody (NB110-39115AF405). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for COX4 Antibody (NB110-39115AF405)

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Pathways for COX4 Antibody (NB110-39115AF405)

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Research Areas for COX4 Antibody (NB110-39115AF405)

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Blogs on COX4.

The Importance of the COX IV Antibody to Apoptosis Research
COX IV isoform 1 is a nuclear-encoded polypeptide chain of the Cytochrome C Oxidase enzyme, located on the mitochondrial inner membrane. Owing to its widespread distribution in human and mammalian tissues, COX IV antibodies are widely used as loading...  Read full blog post.

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Bioinformatics

Gene Symbol COX4I1