COX IV Isoform 2 Antibody (1F2)

Images

 
Western Blot: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Analysis of COX4I2 expression in transfected 293T cell line by COX4I2 monoclonal antibody (M01), clone 1F2.Lane 1: COX4I2 transfected lysate(20 KDa).Lane ...read more
Immunohistochemistry-Paraffin: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Analysis of monoclonal antibody to COX4I2 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
Immunoprecipitation: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Analysis of COX4I2 transfected lysate using anti-COX4I2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with COX4I2 MaxPab rabbit ...read more
ELISA: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Detection limit for recombinant GST tagged COX4I2 is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clone
1F2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

COX IV Isoform 2 Antibody (1F2) Summary

Immunogen
COX4I2 (NP_115998, 21 a.a. ~ 104 a.a) partial recombinant protein with GST tag.MHSSEGTTRGGGKMSPYTNCYAQRYYPMPEEPFCTELNAEEQALKEKEKGSWTQLTHAEKVALYRLQFNETFAEMNRRSNEWKT
Specificity
COX4I2 - cytochrome c oxidase subunit IV isoform 2 (lung)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
COX4I2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Publications
Read Publications using
H00084701-M01 in the following applications:

  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22730437). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for COX IV Isoform 2 Antibody (1F2)

  • COX IV-2
  • COX4
  • COX4-2
  • COX4B
  • COX4-I2
  • COX4L2
  • COXIV-2
  • cytochrome c oxidase subunit 4 isoform 2, mitochondrial
  • cytochrome c oxidase subunit IV isoform 2 (lung)
  • Cytochrome c oxidase subunit IV isoform 2
  • cytochrome c oxidase subunit IV-like 2
  • dJ857M17.2

Background

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes isoform 2 of subunit IV. Isoform 1 of subunit IV is encoded by a different gene, however, the two genes show a similar structural organization. Subunit IV is the largest nuclear encoded subunit which plays a pivotal role in COX regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for COX IV Isoform 2 Antibody (H00084701-M01)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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WB
(2)
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(1)
Mouse
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Showing Publications 1 - 8 of 8.
Publications using H00084701-M01 Applications Species
Schiffer TA, Peleli M, Sundqvist ML et al. Control of human energy expenditure by cytochrome c oxidase subunit IV-2. Am J Physiol Cell Physiol 2016 Sep 1 [PMID: 27486093]
Cloonan SM, Glass K, Laucho-Contreras ME et al. Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Nat Med 2016 Feb [PMID: 26752519]
Aras S, Pak O, Sommer N et al. Oxygen-dependent expression of cytochrome c oxidase subunit 4-2 gene expression is mediated by transcription factors RBPJ, CXXC5 and CHCHD2. Nucleic Acids Res. 2013 Jan 08 [PMID: 23303788]
Morten KJ, Badder L, Knowles HJ. Differential regulation of HIF-mediated pathways increases mitochondrial metabolism and ATP production in hypoxic osteoclasts J Pathol 2013 Apr [PMID: 23303559] (WB, Human) WB Human
Huttemann M, Lee I, Gao X et al. Cytochrome c oxidase subunit 4 isoform 2-knockout mice show reduced enzyme activity, airway hyporeactivity, and lung pathology. FASEB J. 2012 Sep [PMID: 22730437] (WB, Mouse) WB Mouse
Sundar Boyalla S, Barbara Victor M, Roemgens A et al. Sex- and brain region-specific role of cytochrome c oxidase in 1-methyl-4-phenylpyridinium-mediated astrocyte vulnerability. J Neurosci Res. 2011 May 09 [PMID: 21598289]
Misiak M, Singh S, Drewlo S et al. Brain region-specific vulnerability of astrocytes in response to 3-nitropropionic acid is mediated by cytochrome c oxidase isoform expression. Cell Tissue Res 341(1):83-93. 2010 Jul. [PMID: 20602186]
Singh S, Misiak M, Beyer C, Arnold S. Brain region specificity of 3-nitropropionic acid-induced vulnerability of neurons involves cytochrome c oxidase. Neurochem Int 57(3):297-305. 2010 Oct. [PMID: 20600440]

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Bioinformatics

Gene Symbol COX4I2
Entrez
OMIM
Uniprot