COX5A Antibody

Images

 
Western Blot: COX5A Antibody [NBP1-32550] - Sample(30 ug whole cell lysate)A:HeLa S3 B:Hep G2 15% SDS PAGE, antibody diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: COX5A Antibody [NBP1-32550] - A431.
Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550] - Paraffin-embedded mouse cervix. COX5A antibody [N1C3] diluted at 1:500.
Western Blot: COX5A Antibody [NBP1-32550] - Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550] - Paraffin-embedded mouse prostate. COX5A antibody [N1C3] diluted at 1:500.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

COX5A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human COX5A. The exact sequence is proprietary.
Localization
Mitochondrion inner membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COX5A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-32550 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (86%), Rhesus Monkey (97%), Chimpanzee (99%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for COX5A Antibody

  • COX
  • COX-VA
  • Cytochrome c oxidase polypeptide Va
  • cytochrome c oxidase polypeptide, mitochondrial
  • cytochrome c oxidase subunit 5A, mitochondrial
  • cytochrome c oxidase subunit Va
  • mitochondrial cytochrome c oxidase subunit Va
  • VA

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for COX5A Antibody (NBP1-32550)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COX5A
Entrez
Uniprot