Complement Factor B Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Complement Factor B Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Complement Factor B
  Thr26-Leu764 with a Gln34Arg substitution
  Accession # AAA16820
• Specificity: Detects human Complement Factor B in Western blots. In this format, less than 2% cross-reactivity with recombinant human (rh) Factor D and rhFactor I is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CFB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Factor B Antibody [Biotin]

• BF AHUS4
• BF
• B-factor, properdin
• BFD
• C3 proaccelerator
• C3 proactivator
• C3/C5 convertase
• CFAB
• CFB
• Complement Factor B
• EC 3.4.21
• EC 3.4.21.47
• FB
• FBI12
• GBG
• GBGCFAB
• Glycine-rich beta glycoprotein
• glycine-rich beta-glycoprotein
• H2-Bf
• PBF2
• Properdin factor B

Background

Complement Factor B (also C3/C5 convertase, GBC and PBF2) is a 93-95 kDa glycoprotein member of the peptidase S1 family of enzymes.  It is expressed by hepatocytes and macrophages, and serves as a substrate for complement factor D.  Following binding of Factor B to membrane-bound C3b, Factor D acts on Factor B to generate a 33 kDa N-terminal fragment (Ba), plus a 60 kDa C-terminal fragment (Bb) that remains associated with C3b to createe a serine protease.  Mature human Factor B is 739 amino acids (aa) in length (aa 26-764).  It contains three Sushi domains (aa 35-160), one vWFA domain (aa 270-469), and a large peptidase S1 region (aa 477-757).  Cleavage between Arg259Lys260 generates the Ba chain (aa #26-259) and the Bb chain (aa 260-764).  There are two potential isoform variants.   One shows a premature truncation after Gly589, while another shows a 79 aa substitution for aa 543-764.  Over aa 26-764, human shares 85% aa identity with mouse Factor B.        

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CFB
• Uniprot:
  • AAA16820()