Complement C3 Antibody

• Reactivity: Po
• Applications: Flow
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Complement C3 Antibody Summary

• Immunogen: Purified porcine C3
• Clonality: Polyclonal
• Host: Rabbit
• Gene: C3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: For Flow Cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Complement C3 Antibody

• Acylation Stimulating Protein
• acylation-stimulating protein cleavage product
• AHUS5
• ARMD9
• ASP
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
• C3
• C3a anaphylatoxin
• C3a
• C3adesArg
• C3b
• C3bc
• C3-beta-c
• Complement C3 alpha chain
• Complement C3 beta chain
• complement C3
• Complement C3b alpha' chain
• Complement C3c alpha' chain fragment 1
• Complement C3c alpha' chain fragment 2
• Complement C3d fragment
• Complement C3dg fragment
• Complement C3f fragment
• Complement C3g fragment
• complement component 3
• complement component C3
• complement component C3a
• complement component C3b
• CPAMD1
• EC 3.4.21.43
• epididymis secretory sperm binding protein Li 62p
• HEL-S-62p
• prepro-C3

Background

The polyclonal antibody recognizes pig complement component C3. The complement system is an important part of the humoral response in innate immunity, consisting of three different pathways. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. The complement factor C3 consists of an alpha- and a beta-chain.
The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/ml. An inherited deficiency of C3 predisposes the person to frequent assaults of bacterial infections. In ulcerative colitis, and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported.
Proteolysis by certain enzymes results in the cleavage of C3 into C3a and C3b. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Complement C3 Antibody (NBP3-11270). (Showing 1 - 1 of 1 FAQ).

1. I am trying to establish a method to measure mice C3 levels by nephelometry. I would be most grateful if you could provide me with some help, regarding the choice of the Ab. I totally understand that since such a method has never been tried, I do not expect any guaranties. 
   • We have never performed nephelometry in our lab, and do not have a protocol or advice to provide about this application. However, it seems to me that you should choose an antibody that is capable of recognizing its target in its folded conformation. Therefore, I would suggest trying an antibody that has been validated for ICC or IHC.

Bioinformatics

• Gene Symbol: C3