Complement C3 Antibody (11H9) [DyLight 350]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P
Clone
11H9
Clonality
Monoclonal
Host
Rat
Conjugate
DyLight 350

Complement C3 Antibody (11H9) [DyLight 350] Summary

Immunogen
C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1)
Localization
Secreted
Specificity
Mouse Complement C3 and its activation products, C3b, iC3b, C3d and C3dg
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
C3
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
For IHC-F: Tissue sections were fixed in acetone. Antigen retrieval was performed using 50% formic acid for 5 min or microwaved (700 W) in antigen unmasking solution. For IHC-P: free-floating brain tissue pretreated with 50% formic acid for 5 min. or microwaved (700W) in antigen unmasking solution for 2-5min. Blocking endogenous persoxidase by treatment with 3% H2O2 in pBS 20 min at 25 degrees C. Blocking 2hrs, 2% BSA in PBS. For IF: Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Complement C3 Antibody (11H9) [DyLight 350]

  • AHUS5
  • ARMD9
  • ASP
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
  • complement C3
  • complement component 3
  • complement component C3
  • CPAMD1acylation-stimulating protein cleavage product

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Complement C3 Antibody (NB200-540UV). (Showing 1 - 1 of 1 FAQ).

  1. I am trying to establish a method to measure mice C3 levels by nephelometry. I would be most grateful if you could provide me with some help, regarding the choice of the Ab. I totally understand that since such a method has never been tried, I do not expect any guaranties. 
    • We have never performed nephelometry in our lab, and do not have a protocol or advice to provide about this application. However, it seems to me that you should choose an antibody that is capable of recognizing its target in its folded conformation. Therefore, I would suggest trying an antibody that has been validated for ICC or IHC.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Complement C3 Antibody (NB200-540UV)

Discover related pathways, diseases and genes to Complement C3 Antibody (NB200-540UV). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Complement C3.

Complement C3 - the most important protein in the complement system
The complement system is made up of a collection of proteins found in the bloodstream and is comprised of nine major complement proteins; complement C3 is one of them. The complement system is a crucial component of the cellular immune system becau...  Read full blog post.

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Bioinformatics

Gene Symbol C3