Collagen VI Antibody

Images

 
Immunohistochemistry-Frozen: Collagen VI Antibody [NB120-6588] - Feline blood vessels section. The image was captured with an epifluorescent microscope. This image was submitted via customer review.
Immunohistochemistry: Collagen VI Antibody [NB120-6588] - Analysis of Biotin conjugate of NB120-6588. Collagen VI antibody 1:400 45 min RT showed strong staining in FFPE sections of human placenta (Left) with red ...read more
Immunohistochemistry: Collagen VI Antibody [NB120-6588] - Showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Fe, MaSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Collagen VI Antibody Summary

Immunogen
Collagen Type VI from adult human and bovine tissues
Localization
Extracellular matrix.
Specificity
Negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL6A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • ELISA 1:5000-1:50000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:100
Application Notes
Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native PAGE and western blotting for highly sensitive qualitative analysis. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for in immunoblotting. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24378017). Use in FLOW cytometry reported in scientific literature ( PMID 25774777)
Positive Control
Collagen VI Lysate (NBP2-65014)
Reviewed Applications
Read 1 Review rated 4
using
NB120-6588 in the following applications:

Publications
Read Publications using
NB120-6588 in the following applications:

  • 1 publication
  • 1 publication
  • IHC
    2 publications
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.125M Sodium Borate, 75mM NaCl and 5mM EDTA, pH 8.0
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Collagen VI Antibody

  • Collagen 6
  • collagen alpha-1(VI) chain
  • collagen VI, alpha-1 polypeptide
  • collagen, type VI, alpha 1
  • Collagen-6
  • human mRNA for collagen VI alpha-1 C-terminal globular domain10alpha 1 (VI) chain (61 AA)
  • OPLL

Background

This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00001292-M01
Species: Hu, Po
Applications: WB, ChIP, ELISA, ICC/IF
NBP2-34837PEP
Species: Hu
Applications: AC
NBP2-36777
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
MAB1936
Species: Hu
Applications: WB, IP
AF808
Species: Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP2-27561
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP2-30078
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-P
NBP1-88119
Species: Hu
Applications: IHC, IHC-P
H00005688-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, IP
NB600-408
Species: Hu, Mu, Rt, Po, Bv, Fe, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ICC
MAB1419
Species: Hu, Rt
Applications: IHC, CyTOF-ready, ICC, ICFlow
NBP2-14893
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-42388
Species: Hu
Applications: WB, IHC, IHC-P, KD
NB600-594
Species: Hu, Mu, Rt, Bv, Ma
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP1-89953
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NBP1-91258
Species: Hu, Mu, Bv, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-76289
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, ICC
NB120-6588
Species: Hu, Mu, Rt, Bv, Fe, Ma
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for Collagen VI Antibody (NB120-6588)(5)

Review for Collagen VI Antibody (NB120-6588) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Feline.

Reviews using NB120-6588:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Collagen VI NB120-6588
Enlarge
reviewed by:
Anonymous
IHC-Fr Feline 03/09/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedBlood vessels
SpeciesFeline
Lot32613

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen VI Antibody (NB120-6588) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Collagen VI Products

Bioinformatics Tool for Collagen VI Antibody (NB120-6588)

Discover related pathways, diseases and genes to Collagen VI Antibody (NB120-6588). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Collagen VI Antibody (NB120-6588)

Discover more about diseases related to Collagen VI Antibody (NB120-6588).
 

Pathways for Collagen VI Antibody (NB120-6588)

View related products by pathway.

PTMs for Collagen VI Antibody (NB120-6588)

Learn more about PTMs related to Collagen VI Antibody (NB120-6588).
 

Research Areas for Collagen VI Antibody (NB120-6588)

Find related products by research area.

Blogs on Collagen VI

There are no specific blogs for Collagen VI, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
03/09/2018
Application: IHC-Fr
Species: Feline

Bioinformatics

Gene Symbol COL6A1