ENPP-1 Antibody

Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - Negative Control showing staining of paraffin embedded Human Thyroid Gland, with no primary antibody.

Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - Staining of paraffin embedded Human Thyroid Gland at dilution of 8ug/ml. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Rt, Ch, Mu
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ENPP-1 Antibody Summary

• Immunogen: Peptide with sequence C-KTHLPTFSQED corresponding to C-Terminus according to NP_006199.2.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ENPP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8 ug/ml
  • Immunohistochemistry-Paraffin 8 ug/ml
  • Peptide ELISA 1:128000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB reactivity reported in scientific literature (PMID: 20634316).

Reactivity Notes

Rat reactivity reported in (PMID: 17569759). Chicken reactivity reported in scientific literature (PMID: 30271993).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ENPP-1 Antibody

• ectonucleotide pyrophosphatase/phosphodiesterase 1
• E-NPP 1
• ENPP1
• ENPP-1
• Ly-41 Antigen
• M6S1
• M6S1NPP1
• Membrane component chromosome 6 surface marker 1
• membrane component, chromosome 6, surface marker 1
• NPP1
• NPPS
• NPPSalkaline phosphodiesterase 1
• PC1
• PC-1
• PCA1
• PCA1ARHR2
• PDNP1
• PDNP1ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• Phosphodiesterase I/nucleotide pyrophosphatase 1
• plasma-cell membrane glycoprotein 1
• Plasma-cell membrane glycoprotein PC-1

Background

ENPP1 has a broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. It can hydrolyze nucleoside 5' triphosphates such as ATP, GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate. It can also hydrolyze diadenosine polyphosphates and 3',5'-cAMP to AMP. It may play a role in the regulation of pyrophosphate production, the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ENPP-1 Antibody (NBP2-27561)(7)

Publications using NBP2-27561

• Takeda Taka-Aki, Miyazaki Shiho, Kobayashi Miki et al. Zinc deficiency causes delayed ATP clearance and adenosine generation in rats and cell culture models. Frontiers in Immunology 2018 Aug 22 [PMID: 30271993] (WB, Chicken)
• Foster BL, Nagatomo KJ, Nociti FH Jr et al. Central role of pyrophosphate in acellular cementum formation. PLoS One. 2012 [PMID: 22675556] (IHC-P)
  
  Details:
  IHC(p) Bouin's Fixed Tissue (mouse wildtype and ENPP1 knockout mice peridontal tissues): Fig 9. Note: The specificity of the ENPP1 antibody was validated by IHC(P) in ENPP1 wildtype knockout mice (Fig 9).
• Pan W, Ciociola E, Saraf M et al. Metabolic consequences of ENPP1 overexpression in adipose tissue. Am J Physiol Endocrinol Metab. 2011 Nov [PMID: 21810932] (WB, Human)
  
  Details:
  WB: ENPP1 (human) transgenic mice tissue. The specificity of the ENPP1 antibody was validated by WB in ENPP1 (the mice carried a human ENPP1 transgene), see publication for details.
• Lim J, Zhou M, Veenstra TD, Morrison DK. The CNK1 scaffold binds cytohesins and promotes insulin pathway signaling. Genes Dev. 2010 Jul 15 [PMID: 20634316] (WB)
  
  Details:
  WB: Serum starved HepG2 cells stimulated with insulin, Fig 2 & 3.
• Liang J, Fu M, Ciociola E et al. Role of ENPP1 on adipocyte maturation. PLoS One. 2007 Sep 12 [PMID: 17849011] (WB, Human)
  
  Details:
  WB: 3T3-L1 adipocytes transfected with human ENNP1 (Fig 1). Note: The specificity of the ENPP1 antibody was validated by WB in human ENPP1 overexpressing cells (Fig 1). ENPP1 was detected at 130 kDa.
• Orriss IR, Utting JC, Brandao-Burch A et al. Extracellular nucleotides block bone mineralization in vitro: evidence for dual inhibitory mechanisms involving both P2Y2 receptors and pyrophosphate. Endocrinology. 2007 Sep [PMID: 17569759] (WB, Rat)
  
  Details:
  WB: Primary rat osteoblasts (Fig 4B). ENPP-1 protein expression was decreased in osteoblasts treated with ATP, UTP, and PPi compared to untreated osteoblasts. ENPP1 was detected at ~ 150 kDa.
• Meyre D, Bouatia-Naji N, Tounian A et al. Variants of ENPP1 are associated with childhood and adult obesity and increase the risk of glucose intolerance and type 2 diabetes. Nat Genet 2005 Aug [PMID: 16025115]

Bioinformatics

• Gene Symbol: ENPP1
• Uniprot:
  • NP_006199()