ENPP-1 Antibody

Western Blot: ENPP-1 Antibody [NBP2-27561] - HEK293 overexpressing Human ENPP1 and probed with mock transfection in first lane.

Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ENPP-1 Antibody Summary

• Immunogen: Peptide with sequence C-KTHLPTFSQED corresponding to C-Terminus according to NP_006199.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ENPP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 3ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Peptide ELISA 1:32000
• Application Notes: WB: In transfected HEK293 transiently expressing ENPP1 a band of approx. 100 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 105 kDa band according to NP_006199.2. Data obtained from a previous batch (different goat). IHC-P: Human liver shows membranous staining in many hepatocytes. WB reactivity reported in scientific literature (PMID: 20634316).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ENPP-1 Antibody

• ectonucleotide pyrophosphatase/phosphodiesterase 1
• E-NPP 1
• ENPP1
• ENPP-1
• Ly-41 Antigen
• M6S1
• M6S1NPP1
• Membrane component chromosome 6 surface marker 1
• membrane component, chromosome 6, surface marker 1
• NPP1
• NPPS
• NPPSalkaline phosphodiesterase 1
• PC1
• PC-1
• PCA1
• PCA1ARHR2
• PDNP1
• PDNP1ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• Phosphodiesterase I/nucleotide pyrophosphatase 1
• plasma-cell membrane glycoprotein 1
• Plasma-cell membrane glycoprotein PC-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ENPP-1 Antibody (NBP2-27561)(6)

Publications using NBP2-27561

• Foster BL, Nagatomo KJ, Nociti FH Jr et al. Central role of pyrophosphate in acellular cementum formation. PLoS One. 2012 [PMID: 22675556] (IHC-P)
  
  Details:
  IHC(p) Bouin's Fixed Tissue (mouse wildtype and ENPP1 knockout mice peridontal tissues): Fig 9. Note: The specificity of the ENPP1 antibody was validated by IHC(P) in ENPP1 wildtype knockout mice (Fig 9).
• Pan W, Ciociola E, Saraf M et al. Metabolic consequences of ENPP1 overexpression in adipose tissue. Am J Physiol Endocrinol Metab. 2011 Nov [PMID: 21810932] (WB, Human)
  
  Details:
  WB: ENPP1 (human) transgenic mice tissue. The specificity of the ENPP1 antibody was validated by WB in ENPP1 (the mice carried a human ENPP1 transgene), see publication for details.
• Lim J, Zhou M, Veenstra TD, Morrison DK. The CNK1 scaffold binds cytohesins and promotes insulin pathway signaling. Genes Dev. 2010 Jul 15 [PMID: 20634316] (WB)
  
  Details:
  WB: Serum starved HepG2 cells stimulated with insulin, Fig 2 & 3.
• Liang J, Fu M, Ciociola E et al. Role of ENPP1 on adipocyte maturation. PLoS One. 2007 Sep 12 [PMID: 17849011] (WB, Human)
  
  Details:
  WB: 3T3-L1 adipocytes transfected with human ENNP1 (Fig 1). Note: The specificity of the ENPP1 antibody was validated by WB in human ENPP1 overexpressing cells (Fig 1). ENPP1 was detected at 130 kDa.
• Orriss IR, Utting JC, Brandao-Burch A et al. Extracellular nucleotides block bone mineralization in vitro: evidence for dual inhibitory mechanisms involving both P2Y2 receptors and pyrophosphate. Endocrinology. 2007 Sep [PMID: 17569759] (WB, Rat)
  
  Details:
  WB: Primary rat osteoblasts (Fig 4B). ENPP-1 protein expression was decreased in osteoblasts treated with ATP, UTP, and PPi compared to untreated osteoblasts. ENPP1 was detected at ~ 150 kDa.
• Meyre D, Bouatia-Naji N, Tounian A et al. Variants of ENPP1 are associated with childhood and adult obesity and increase the risk of glucose intolerance and type 2 diabetes. Nat Genet 2005 Aug [PMID: 16025115]

Bioinformatics

• Gene Symbol: ENPP1
• Entrez:
  • 5167(Human)
• Uniprot:
  • NP_006199(Human)