Hu,
Mu,
Rt,
Bv,
FeSpecies Glossary
Reactivity: Hu, Mu, Rt, Bv, Fe
WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Func
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Func
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: Collagen VI alpha 1 Antibody [NB120-6588] - Purification and imaging of collagen VI microfibrils from bovine cornea. Reducing SDS-PAGE (left hand panel) and western blot (right hand panel) of the central fraction of the void peak. Collagen VI chains were detected using a polyclonal rabbit anti collagen VI antibody. Arrows highlight bands at approximately 250 kDa, which corresponds to the alpha3 chain, and at 120 kDa which corresponds to alpha1 and alpha2 chains. Image collected and cropped by CiteAb from the following publication (www.linkinghub.elsevier.com/retrieve/pii/S1742706116306912) licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Collagen-VI-alpha-1-Antibody-Western-Blot-NB120-6588-img0007.jpg "Western Blot: Collagen VI alpha 1 Antibody [NB120-6588] - Purification and imaging of collagen VI microfibrils from bovine cornea. Reducing SDS-PAGE (left hand panel) and western blot (right hand panel) of the central fraction of the void peak. Collagen VI chains were detected using a polyclonal rabbit anti collagen VI antibody. Arrows highlight bands at approximately 250 kDa, which corresponds to the alpha3 chain, and at 120 kDa which corresponds to alpha1 and alpha2 chains. Image collected and cropped by CiteAb from the following publication (www.linkinghub.elsevier.com/retrieve/pii/S1742706116306912) licensed under a CC-BY licence.")
![Immunohistochemistry-Frozen: Collagen VI alpha 1 Antibody [NB120-6588] - Feline blood vessels section. The image was captured with an epifluorescent microscope. Image submitted by a verified customer review](http://images.novusbio.com/fullsize2/Collagen-VI-alpha-1-Antibody-Immunohistochemistry-Frozen-NB120-6588-img0006.jpg)
![Flow Cytometry: Collagen VI alpha 1 Antibody [NB120-6588] - Effect of PTX3 on morphology, collagen production, & receptor expression. After 5 days incubation with 1 ug/ml PTX3, PBMCs were air-dried, fixed, & stained with anti-collagen-I or control antibodies. (Positive staining - red staining; nuclei - blue). Bar is 50 um. Following ICC staining, at least 100 elongated cells with oval nuclei were examined from at least 10 randomly selected fields, & the % of positive cells is expressed as mean +/- SEM (n = 3-5 donors). Supernatants from PBMC incubated in the absence (-) or presence (+) of 1 ug/ml PTX3 assessed by western blot. Adherent cells (macrophages & fibrocytes) were stained with collagen-VI (teal line SFM, red +PTX3) or control IgY (black SFM, orange +PTX3) antibodies. The data are representative of three separate experiments. Image collected & cropped by CiteAb from the following publication (www.dx.plos.org/10.1371/journal.pone.0119709) licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Collagen-VI-alpha-1-Antibody-Flow-Cytometry-NB120-6588-img0008.jpg)
![Immunohistochemistry: Collagen VI alpha 1 Antibody [NB120-6588] - Showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval. Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize2/Collagen-VI-alpha-1-Antibody-Immunohistochemistry-NB120-6588-img0005.jpg)
![Western Blot: Collagen VI alpha 1 Antibody [NBP2-15947] - Wild-type (WT) and COL6A1 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Collagen-VI-alpha-1-Antibody-Western-Blot-NBP2-15947-img0009.jpg "Western Blot: Collagen VI alpha 1 Antibody [NBP2-15947] - Wild-type (WT) and COL6A1 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody [NBP2-15947] - Detects COL6A1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Collagen VI alpha 1 stained by diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize2/Collagen-VI-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15947-img0008.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP2-15947] - Mouse liver. COL6A1 antibody [C1C2], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize2/Collagen-VI-Antibody-Immunohistochemistry-Paraffin-NBP2-15947-img0005.jpg)
![Western Blot: Collagen VI alpha 1 Antibody [NBP2-15947] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with COL6A1 antibody [C1C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Collagen-VI-Antibody-Western-Blot-NBP2-15947-img0007.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP2-15947] - Human hepatoma. COL6A1 antibody [C1C2], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize2/Collagen-VI-Antibody-Immunohistochemistry-Paraffin-NBP2-15947-img0003.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Staining of human placenta shows moderate positivity in extracellular matrix.](http://images.novusbio.com/fullsize2/Collagen-VI-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP1-91193-img0010.jpg "Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Staining of human placenta shows moderate positivity in extracellular matrix.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Analysis in human placenta and pancreas tissues. Corresponding COL6A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Collagen-VI-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP1-91193-img0006.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Staining of human testis shows weak positivity in extracellular matrix in peritubular myoid cells.](http://images.novusbio.com/fullsize2/Collagen-VI-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP1-91193-img0007.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Staining of human skin shows moderate positivity in extracellular matrix.](http://images.novusbio.com/fullsize2/Collagen-VI-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP1-91193-img0008.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize2/Collagen-VI-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP1-91193-img0009.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91195] - Collagen VI Antibody [NBP1-91195] - Staining in human gallbladder and pancreas tissues using anti-COL6A1 antibody. Corresponding COL6A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Collagen-VI-Antibody-Immunohistochemistry-Paraffin-NBP1-91195-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91195] - Collagen VI Antibody [NBP1-91195] - Staining in human gallbladder and pancreas tissues using anti-COL6A1 antibody. Corresponding COL6A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Collagen VI Antibody [NBP1-91195] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize2/Collagen-VI-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91195-img0005.jpg)
![Immunohistochemistry-Paraffin: Collagen VI Antibody [NBP1-91195] - Staining of human gallbladder shows high expression.](http://images.novusbio.com/fullsize2/Collagen-VI-Antibody-Immunohistochemistry-Paraffin-NBP1-91195-img0006.jpg)
![Immunohistochemistry-Paraffin: Collagen VI Antibody [NBP1-91195] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize2/Collagen-VI-Antibody-Immunohistochemistry-Paraffin-NBP1-91195-img0007.jpg)
![Western Blot: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869] - Western blot analysis of extracts of various cell lines, using Collagen VI alpha 1 Rabbit mAb (NBP3-16869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize2/Collagen-VI-alpha-1-Antibody-(7J1P4)-Western-Blot-NBP3-16869-img0003.jpg "Western Blot: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869] - Western blot analysis of extracts of various cell lines, using Collagen VI alpha 1 Rabbit mAb (NBP3-16869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869] - Immunohistochemistry of paraffin-embedded human liver using Collagen VI alpha 1 Rabbit mAb (NBP3-16869) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize2/Collagen-VI-alpha-1-Antibody-(7J1P4)-Immunohistochemistry-Paraffin-NBP3-16869-img0002.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869] - Immunohistochemistry of paraffin-embedded rat lung using Collagen VI alpha 1 Rabbit mAb (NBP3-16869) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize2/Collagen-VI-alpha-1-Antibody-(7J1P4)-Immunohistochemistry-Paraffin-NBP3-16869-img0001.jpg)
![Western Blot: Collagen VI Antibody [NBP1-59126] - Titration: 5% Milk, Positive Control: human LCL.](http://images.novusbio.com/fullsize2/Collagen-VI-Antibody-Western-Blot-NBP1-59126-img0004.jpg "Western Blot: Collagen VI Antibody [NBP1-59126] - Titration: 5% Milk, Positive Control: human LCL.")
![Western Blot: Collagen VI Antibody [NBP1-59126] - Titration: 0.2-1 ug/ml, Positive Control: 721_B cell lysate.](http://images.novusbio.com/fullsize2/Collagen-VI-Antibody-Western-Blot-NBP1-59126-img0003.jpg)
![SDS-Page: Recombinant Human Collagen VI Protein [H00001291-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001291-Q01-1.jpg "SDS-Page: Recombinant Human Collagen VI Protein [H00001291-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
-img0001.jpg "Human Collagen VI alpha 1 ELISA Kit (Colorimetric) - Standard Curve Reference")
![Western Blot: Collagen VI Overexpression Lysate (Adult Normal) [NBP2-10777] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Collagen VI.](http://images.novusbio.com/fullsize/Collagen-VI-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBP2-10777-img0001.jpg "Western Blot: Collagen VI Overexpression Lysate (Adult Normal) [NBP2-10777] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Collagen VI.")
-img0001.jpg "Human Collagen VI alpha 1 ELISA Kit (Chemiluminescence) - Standard Curve Reference")