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Collagen VI alpha 2 Antibody (2C5-F2)

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Western Blot: Collagen VI alpha 2 Antibody (2C5-F2) [H00001292-M01] - Analysis of COL6A2 expression in transfected 293T cell line by COL6A2 monoclonal antibody (M01), clone 2C5-F2.Lane 1: COL6A2 transfected lysate(109 KDa).Lane 2: Non-transfected lysate.
Western Blot: Collagen VI alpha 2 Antibody (2C5-F2) [H00001292-M01] - Analysis of COL6A2 expression in transfected 293T cell line by COL6A2 monoclonal antibody (M01), clone 2C5-F2.Lane 1: COL6A2 transfected lysate(109 ...read more
Immunocytochemistry/ Immunofluorescence: Collagen VI alpha 2 Antibody (2C5-F2) [H00001292-M01] - IF staining of Collagen VI alpha 2 in Pig Trabecular meshwork cells using anti-Collagen VI alpha 2 antibody. Image from ...read more

Product Details

Summary
Reactivity Hu, PoSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
2C5-F2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Collagen VI alpha 2 Antibody (2C5-F2) Summary

Immunogen
COL6A2 (AAH02484, 1 a.a. ~ 425 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDEHIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYDRLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCDRDVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTELSVAQCTQRPVDIVFLLDGSERLGEQNFHKARRFVEQVARRLTLARRDDDPLNARVALLQFGGPGEQQVAFPLSHNLTAIHEALETTQYLNSFSHVGAGVVHAINAIVRSPRGGARRHAELSFVFLTDGVTGNDSLHESAHSMRKQNVVPTVLALGSDVDMDVLTTLSLGDRAAVFHEKDYDSLAQPGFFDRFIRWIC
Specificity
COL6A2 - collagen, type VI, alpha 2
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
COL6A2
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA. ICC/IF reactivity per customer review.
Reviewed Applications
Read 1 Review rated 5
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H00001292-M01 in the following applications:
Publications
Read Publication using H00001292-M01.

Reactivity Notes

Porcine reactivity per customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Collagen VI alpha 2 Antibody (2C5-F2)

  • collagen alpha-2(VI) chain
  • collagen VI, alpha-2 polypeptide
  • collagen, type VI, alpha 2,10PP3610
  • DKFZp586E1322
  • FLJ46862

Background

This gene encodes one of the three alpha chains of type VI collagen, a beaded filament collagen found in most connective tissues. The product of this gene contains several domains similar to von Willebrand Factor type A domains. These domains have been shown to bind extracellular matrix proteins, an interaction that explains the importance of this collagen in organizing matrix components. Mutations in this gene are associated with Bethlem myopathy and Ullrich scleroatonic muscular dystrophy. Three transcript variants have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: Collagen VI alpha 1 Antibody [NB120-6588] - Showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval. Image using the Biotin format of this antibody.
Immunohistochemistry-Frozen: Collagen VI alpha 1 Antibody [NB120-6588] - Feline blood vessels section. The image was captured with an epifluorescent microscope. Image submitted by a verified customer review
Flow Cytometry: Collagen VI alpha 1 Antibody [NB120-6588] - Effect of PTX3 on morphology, collagen production, & receptor expression. After 5 days incubation with 1 ug/ml PTX3, PBMCs were air-dried, fixed, & stained with anti-collagen-I or control antibodies. (Positive staining - red staining; nuclei - blue). Bar is 50 um. Following ICC staining, at least 100 elongated cells with oval nuclei were examined from at least 10 randomly selected fields, & the % of positive cells is expressed as mean +/- SEM (n = 3-5 donors). Supernatants from PBMC incubated in the absence (-) or presence (+) of 1 ug/ml PTX3 assessed by western blot. Adherent cells (macrophages & fibrocytes) were stained with collagen-VI (teal line SFM, red +PTX3) or control IgY (black SFM, orange +PTX3) antibodies. The data are representative of three separate experiments. Image collected & cropped by CiteAb from the following publication (www.dx.plos.org/10.1371/journal.pone.0119709) licensed under a CC-BY licence.
(1 Review)
(14 Publications)
Species: Bv, Fe, Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PCR, WB
Collagen VI alpha
3 Antibody
NBP2-97126
Western Blot: Collagen VI alpha 3 Antibody [NBP2-97126] - Anti-Collagen VI alpha 3 rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH-3T3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 108 kDa. Observed band size: 108 kDa (We are unsure as to the identity of these extra bands).w
Species: Hu
Applications: IHC, IHC-P, IP, WB
Collagen III alpha
1/COL3A1 Antibody
NB600-594
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C. 20 minutes for antigen retrieval.
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: FFPE. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:500 for 4 degrees Celsius for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type III is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.
Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Western blot for COL3A1 protein with PVDF protein staining to control for protein loading. Image collected and cropped by CiteAb from the following publication (mdpi.com/2076-3921/6/4/87), licensed under a CC-BY licence.
(7 Reviews)
(31 Publications)
Species: Bv, Fe, Hu, Mu, Rt, Sh
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
COL1A2 Antibody
NBP2-92790
Western Blot: COL1A2 Antibody [NBP2-92790] - Analysis of extracts of A375 cells, using Collagen I/COL1A2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: COL1A2 Antibody [NBP2-92790] - Human placenta using Collagen I/COL1A2 Rabbit pAb (A5786) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunocytochemistry/Immunofluorescence: COL1A2 Antibody [NBP2-92790] - Analysis of A-431 cells using Collagen I/COL1A2 antibody at dilution of 1:25. Blue: DAPI for nuclear staining.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Collagen I Antibody
NB600-408
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Balb/3T3 mouse embryonic fibroblast cell line stained with Collagen I antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Mouse pancreas tissue stained with Collagen I antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Human lung tissue section stained with Collagen I antibody at 1:400.
(16 Reviews)
(237 Publications)
Species: Bv, Hu, Mu, Po, Rb, Rt
Applications: ELISA, FLISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, MiAr, PAGE, WB
S100B Antibody
(15F9NB) - BSA Free
NBP2-45267
Flow Cytometry: S100B Antibody (15F9NB) [NBP2-45267] - An intracellular stain was performed on SK-MEL-28 cells with S100B [15F9NB] Antibody NBP2-45267PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Flow Cytometry: S100B Antibody (15F9NB) [NBP2-45267] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (Orange) stained at a dilution of 1:500. Detected with a GtxRb DyLight 488 secondary. Shown with the secondary control (Blue).
Immunocytochemistry/Immunofluorescence: S100B Antibody (15F9NB) [NBP2-45267] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
SPARC Antibody
[Unconjugated]
AF942
Western blot shows lysates of C2C12 mouse myoblast cell line and mouse placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SPARC at approximately 35-37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SPARC/Osteonectin was detected in immersion fixed frozen sections of mouse embryo (E15) using Mouse SPARC/Osteonectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing cartilage. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
SPARC was detected in immersion fixed C2C12 mouse myoblast cell line using Goat Anti-Mouse SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endoplasmic reticuli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Fibronectin
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NBP1-91258
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Feline corneal stromal cells. IHC-Fr and IF (Alexa Fluor488) were performed using NBP1-91258 at 1:400. Image captured by epifluorescent microscopy. Image from verified customer review.
Western Blot: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate.
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Trabecular meshwork (TM) region of pig eyes. NBP1-91258 Fibronectin antibody was labelled with Alexa Fluor 488 conjugated secondary antibody (Green). DAPI shown as blue. Image from verified customer review.
(11 Reviews)
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Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
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FKRP Antibody
NBP3-04945
Western Blot: FKRP Antibody [NBP3-04945] - Analysis of extracts of various cell lines, using FKRP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
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Applications: IHC, IHC-P, WB
FKTN Antibody
(7J5N6)
NBP3-15472
Western Blot: FKTN Antibody (7J5N6) [NBP3-15472] - Western blot analysis of extracts of various cell lines, using FKTN antibody (NBP3-15472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry-Paraffin: FKTN Antibody (7J5N6) [NBP3-15472] - Immunohistochemistry of paraffin-embedded human colon using FKTN Rabbit mAb (NBP3-15472) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Endostatin [HRP]
DNST0
(22 Publications)
Species: Hu
Applications: ELISA
hnRNP C1 + C2
Antibody (CL2593)
NBP2-36776
Western Blot: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HNRNPC antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunohistochemistry: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining of human rectum shows strong nuclear immunoreactivity in glandular and lamina propria cells.
Immunohistochemistry: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining of human small intestine shows strong nuclear positivity in glandular epithelium and in lamina propria cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
SEPN1 Antibody
NBP2-34098
Immunohistochemistry-Paraffin: SEPN1 Antibody [NBP2-34098] - Staining of human lung shows strong cytoplasmic positivity in pneumocytes and macrophages.
Immunocytochemistry/Immunofluorescence: SEPN1 Antibody [NBP2-34098] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Collagen V alpha 2
Antibody (3G11)
H00001290-M02
ELISA: Collagen V alpha 2 Antibody (3G11) [H00001290-M02] - Detection limit for recombinant GST tagged COL5A2 is 0.03 ng/ml as a capture antibody.
Immunocytochemistry/Immunofluorescence: Collagen V alpha 2 Antibody (3G11) [H00001290-M02] - Analysis of monoclonal antibody to COL5A2 on HeLa cell . Antibody concentration 10 ug/ml.
Species: Hu
Applications: ELISA, ICC/IF, WB
Collagen V
Antibody (1E2-E4/Col5 ...
NBP1-05118
Western Blot: Collagen V Antibody (1E2-E4/Col5) [NBP1-05118] - Staining with 1E2-E4/Col5 on Dog Type I, III, V and Human Type I, III, and V in rows 1-6 respectively. (1. Werkmeister)
(2 Publications)
Species: Bv, Ca, Ch(-), Gp(-), Hu, Ma, Mu(-), Po, Rb, Rt(-), Sh
Applications: ELISA, IHC, IHC-Fr, IHC-P, WB
Fibulin 1 Antibody
NBP1-84725
Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Analysis in human placenta and skeletal muscle tissues using NBP1-84725 antibody. Corresponding FBLN1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Staining of human endometrium shows moderate positivity in extracellular matrix.
(1 Review)
(6 Publications)
Species: Hu
Applications: IHC, IHC-P, WB
Peripherin 2/PRPH2
Antibody
NBP1-86687
Western Blot: Peripherin 2/PRPH2 Antibody [NBP1-86687] - Analysis in control (vector only transfected HEK293T lysate) and PRPH2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Peripherin 2/PRPH2 Antibody [NBP1-86687] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: Peripherin 2/PRPH2 Antibody [NBP1-86687] - Staining of human endometrium shows no positivity in glandular cells as expected.
Species: Hu
Applications: IHC, IHC-P, WB
RanGAP1 Antibody
(OTI1B4)
NBP2-02623
Flow Cytometry: RanGAP1 Antibody (1B4) [NBP2-02623] - Analysis of Hela cells, using anti-RanGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: RanGAP1 Antibody (1B4) [NBP2-02623] - Analysis of Jurkat cells, using anti-RanGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: RanGAP1 Antibody (1B4) [NBP2-02623] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RanGAP1 antibody, and then analyzed by flow cytometry.
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
SPARC-like
1/SPARCL1 Antibody [U ...
AF2728
SPARC‑like 1/SPARCL1 was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human SPARC‑like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SPARC‑like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SPARC‑like 1/SPARCL1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HL‑60 human acute promyelocytic leukemia cell line was stained with Goat Anti-Human SPARC‑like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).  To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. 
(5 Publications)
Species: Hu
Applications: CyTOF-ready, IHC, ICFlow, WB
Collagen VI alpha
2 Antibody (2C5-F2)
H00001292-M01
Collagen VI alpha 2 Antibody (2C5-F2)
Species: Hu, Po
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Collagen VI alpha 2 Antibody (H00001292-M01)(1)


Review for Collagen VI alpha 2 Antibody (H00001292-M01) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using H00001292-M01:
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Immunocytochemistry Collagen VI alpha 2 H00001292-M01
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reviewed by:
Anonymous -
 ICC Other 02/02/2016
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Summary

ApplicationImmunocytochemistry
Sample TestedPig trabecular meshwork cells
SpeciesOther

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Immunocytochemistry Collagen VI alpha 2 H00001292-M01
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 Anonymous -
02/02/2016
Application: ICC
Species: Other
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Bioinformatics

Gene Symbol COL6A2
Entrez
OMIM
Uniprot