Collagen VI alpha 1 Antibody

Images

 
Immunohistochemistry-Frozen: Collagen VI alpha 1 Antibody [NB120-6588] - Feline blood vessels section. The image was captured with an epifluorescent microscope. Image submitted by a verified customer review
Immunohistochemistry: Collagen VI alpha 1 Antibody [NB120-6588] - Showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Fe, MaSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Func
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Collagen VI alpha 1 Antibody Summary

Immunogen
Collagen Type VI from human and bovine placenta. Uniprot ID P12109
Localization
Extracellular matrix.
Specificity
Negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL6A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • ELISA 1:5000-1:50000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:100
  • PCR
Application Notes
Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native PAGE and western blotting for highly sensitive qualitative analysis. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for in immunoblotting. Use in ICC/IF reported in scientific literature (PMID 24378017). Use in FLOW cytometry reported in scientific literature ( PMID 25774777). Collagen VI alpha 1 antibody validated for IHC-F from a verified customer review. Use in PCR reported in secitific publication PMID: 32719790
Control
Collagen VI alpha 1 Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB120-6588 in the following applications:

Publications
Read Publications using
NB120-6588 in the following applications:

  • 1 publication
  • 2 publications
  • IHC
    5 publications
  • 1 publication
  • PCR
    1 publication
  • WB
    4 publications

Reactivity Notes

This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reactivity of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Collagen VI alpha 1 Antibody

  • Collagen 6
  • collagen alpha-1(VI) chain
  • collagen VI, alpha-1 polypeptide
  • collagen, type VI, alpha 1
  • Collagen-6
  • human mRNA for collagen VI alpha-1 C-terminal globular domain10alpha 1 (VI) chain (61 AA)
  • OPLL

Background

This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen VI alpha 1 Antibody (NB120-6588)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: FLOW, ICC/IF, IHC, IHC-P, PCR, WB.


Filter By Application
FLOW
(1)
ICC/IF
(2)
IHC
(5)
IHC-P
(1)
PCR
(1)
WB
(4)
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Filter By Species
Human
(6)
Mouse
(4)
Rat
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB120-6588 Applications Species
MondragOn E, Cowdin M, Taraballi F et al. Mimicking the Organic and Inorganic Composition of Anabolic Bone Enhances Human Mesenchymal Stem Cell Osteoinduction and Scaffold Mechanical Properties Front Bioeng Biotechnol Jul 3 2020 [PMID: 32719790] (ICC/IF, PCR, Human) ICC/IF, PCR Human
De Luca M, Vecchie' D, Athmanathan B et al. Genetic Deletion of Syndecan-4 Alters Body Composition, Metabolic Phenotypes, and the Function of Metabolic Tissues in Female Mice Fed A High-Fat Diet Nutrients Nov 18 2019 [PMID: 31752080] (IHC-P, Mouse) IHC-P Mouse
Barbariga M, Vallone F, Mosca E et al. The role of extracellular matrix in mouse and human corneal neovascularization Sci Rep 2019 Oct 03 [PMID: 31582785] (WB, Human, Mouse) WB Human, Mouse
Lionello VM, Nicot AS, Sartori M et al. Amphiphysin 2 modulation rescues myotubular myopathy and prevents focal adhesion defects in mice Sci Transl Med Mar 20 2019 [PMID: 30894500] (IHC, Mouse) IHC Mouse
Angenendt L, Mikesch JH, Gorlich D et al. Stromal collagen type VI associates with features of malignancy and predicts poor prognosis in salivary gland cancer Cell Oncol (Dordr) Jun 15 2018 [PMID: 29949051] (IHC, Human) IHC Human
Thomas-Porch C, Li J, Zanata F et al. Comparative Proteomic Analyses of Human Adipose Extracellular Matrices Decellularized Using Alternative Procedures J Biomed Mater Res A Apr 25 2018 [PMID: 29693792] (IHC, Human) IHC Human
Godwin AR, Starborg T, Sherratt MJ et al. Defining the hierarchical organisation of collagen VI microfibrils at nanometre to micrometre length scales. Acta Biomater. Dec 10 2016 [PMID: 27956360] (WB, Human) WB Human
Nygaard RH, Maynard S, Schjerling P et al. Acquired Localized Cutis Laxa due to Increased Elastin Turnover. Case Rep Dermatol 2016 Jan-Apr [PMID: 27293393] (WB) WB
Izu Y, Ezura Y, Koch M et al. Collagens VI and XII form complexes mediating osteoblast interactions during osteogenesis. Cell Tissue Res. 2016 Jan 12 [PMID: 26753503] (WB, Mouse) WB Mouse
Pilling D, Cox N, Vakil V et al. The Long Pentraxin PTX3 Promotes Fibrocyte Differentiation. PLoS ONE. 2015 Mar 17 [PMID: 25774777] (IHC, FLOW, Human) IHC, FLOW Human
Show All 11 Publications.

Review for Collagen VI alpha 1 Antibody (NB120-6588) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Feline.

Reviews using NB120-6588:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Collagen VI alpha 1 NB120-6588
Enlarge
4
reviewed by:
Anonymous
IHC-Fr Feline 03/09/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedBlood vessels
SpeciesFeline
Lot32613

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen VI alpha 1 Antibody (NB120-6588) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin NB120-6583
DyLight 594 NB120-6588DL594

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Recent Reviews

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4
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2
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1
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Anonymous
03/09/2018
Application: IHC-Fr
Species: Feline

Bioinformatics

Gene Symbol COL6A1