Collagen VI alpha 1 Antibody

Immunohistochemistry-Frozen: Collagen VI alpha 1 Antibody [NB120-6588] - Feline blood vessels section. The image was captured with an epifluorescent microscope. Image submitted by a verified customer review

Immunohistochemistry: Collagen VI alpha 1 Antibody [NB120-6588] - Showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval. Image using the Biotin format of this antibody.

• Reactivity: Hu, Mu, Rt, Bv, Fe, Ma
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Collagen VI alpha 1 Antibody Summary

• Immunogen: Collagen Type VI from human and bovine placenta. Uniprot ID P12109
• Localization: Extracellular matrix.
• Specificity: Negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL6A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:10000
  • ELISA 1:5000-1:50000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:100
• Application Notes: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native PAGE and western blotting for highly sensitive qualitative analysis. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for in immunoblotting. Use in ICC/IF reported in scientific literature (PMID 24378017). Use in FLOW cytometry reported in scientific literature ( PMID 25774777). Collagen VI alpha 1 antibody validated for IHC-F from a verified customer review.
• Control:

  Collagen VI alpha 1 Knockout 293T Cell Lysate

Reactivity Notes

This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reactivity of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Collagen VI alpha 1 Antibody

• Collagen 6
• collagen alpha-1(VI) chain
• collagen VI, alpha-1 polypeptide
• collagen, type VI, alpha 1
• Collagen-6
• human mRNA for collagen VI alpha-1 C-terminal globular domain10alpha 1 (VI) chain (61 AA)
• OPLL

Background

This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen VI alpha 1 Antibody (NB120-6588)(10)

Publications using NB120-6588

• De Luca M, Vecchie' D, Athmanathan B et al. Genetic Deletion of Syndecan-4 Alters Body Composition, Metabolic Phenotypes, and the Function of Metabolic Tissues in Female Mice Fed A High-Fat Diet Nutrients Nov 18 2019 [PMID: 31752080] (IHC-P, Mouse)
• Barbariga M, Vallone F, Mosca E et al. The role of extracellular matrix in mouse and human corneal neovascularization Sci Rep 2019 Oct 03 [PMID: 31582785] (WB, Human, Mouse)
• Lionello VM, Nicot AS, Sartori M et al. Amphiphysin 2 modulation rescues myotubular myopathy and prevents focal adhesion defects in mice Sci Transl Med Mar 20 2019 [PMID: 30894500] (IHC, Mouse)
• Angenendt L, Mikesch JH, Gorlich D et al. Stromal collagen type VI associates with features of malignancy and predicts poor prognosis in salivary gland cancer Cell Oncol (Dordr) Jun 15 2018 [PMID: 29949051] (IHC, Human)
• Thomas-Porch C, Li J, Zanata F et al. Comparative Proteomic Analyses of Human Adipose Extracellular Matrices Decellularized Using Alternative Procedures J Biomed Mater Res A Apr 25 2018 [PMID: 29693792] (IHC, Human)
• Godwin AR, Starborg T, Sherratt MJ et al. Defining the hierarchical organisation of collagen VI microfibrils at nanometre to micrometre length scales. Acta Biomater. Dec 10 2016 [PMID: 27956360] (WB, Human)
• Nygaard RH, Maynard S, Schjerling P et al. Acquired Localized Cutis Laxa due to Increased Elastin Turnover. Case Rep Dermatol 2016 Jan-Apr [PMID: 27293393] (WB)
• Izu Y, Ezura Y, Koch M et al. Collagens VI and XII form complexes mediating osteoblast interactions during osteogenesis. Cell Tissue Res. 2016 Jan 12 [PMID: 26753503] (WB, Mouse)
• Pilling D, Cox N, Vakil V et al. The Long Pentraxin PTX3 Promotes Fibrocyte Differentiation. PLoS ONE. 2015 Mar 17 [PMID: 25774777] (IHC, FLOW, Human)
• Scarritt ME, Bonvillain RW, Burkett BJ et al. HYPERTENSIVE RAT LUNGS RETAIN HALLMARKS OF VASCULAR DISEASE UPON DECELLULARIZATION BUT SUPPORT THE GROWTH OF MESENCHYMAL STEM CELLS. Tissue Eng Part A 2013 Dec 31 [PMID: 24378017] (IHC, ICC/IF, Rat)

Review for Collagen VI alpha 1 Antibody (NB120-6588) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Feline.

reviewed by: Anonymous IHC-Fr Feline 03/09/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Blood vessels
• Species: Feline
• Lot: 32613

Bioinformatics

• Gene Symbol: COL6A1