Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [Biotin] [NB120-6581] - Immunohistochemical staining of Collagen IV alpha 1 (red) and Insulin (green) in mouse pancreas. Image from verified customer review.
Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [Biotin] [NB120-6581] - collagen IV antibody 1:400, 45 min RT showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed ...read more
The development of type-specific antibodies is dependent on NON-DENATURED three-dimensional epitopes.
Secreted and Cell surface (Potential).
Negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Biotin (A=N/A, E=N/A)
0.125 M Sodium Borate (pH 8.0), 0.075 M NaCl, and 0.005 M EDTA
0.01% Sodium Azide
Immunogen affinity purified
Reconstitute with 0.1 ml deionized water.
Western Blot 1:2000-1:10000
Immunohistochemistry-Paraffin 1:1000 - 1:5000
This product was assayed by immunoblot and found to be reactive against Collagen IV at a dilution of 1:5,000 - 1:10,000. This product was also assayed against 1.0 ug of Collagen IV in a standard sandwich ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 - 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections, dilute the product 1:50 - 1:200.
Read 1 Review rated 5 using NB120-6581 in the following applications:
This antibody is expected to react with Type IV collagens from most mammalian species. Rat reactivity reported in scientific literature (PMID: 16774905)
Alternate Names for Collagen IV alpha 1 Antibody [Biotin]
COL4A1 NC1 domain
collagen alpha-1(IV) chain
collagen IV, alpha-1 polypeptide
collagen, type IV, alpha 1
These antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.