Collagen IV alpha 1 Antibody [Biotin]

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Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [Biotin] [NB120-6581] - Immunohistochemical staining of Collagen IV alpha 1 (red) and Insulin (green) in mouse pancreas. Image from verified customer review.
Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [Biotin] [NB120-6581] - collagen IV antibody 1:400, 45 min RT showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, MaSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin
Concentration
LYOPH

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Collagen IV alpha 1 Antibody [Biotin] Summary

Immunogen
Collagen Type IV from human and bovine placenta.
Epitope
The development of type-specific antibodies is dependent on NON-DENATURED three-dimensional epitopes.
Localization
Secreted and Cell surface (Potential).
Specificity
Negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL4A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • ELISA 1:20000-1:100000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 1:100
Application Notes
This product was assayed by immunoblot and found to be reactive against Collagen IV at a dilution of 1:5,000 - 1:10,000. This product was also assayed against 1.0 ug of Collagen IV in a standard sandwich ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 - 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections, dilute the product 1:50 - 1:200.
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Reviewed Applications
Read 1 Review rated 5
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NB120-6581 in the following applications:

Reactivity Notes

This antibody is expected to react with Type IV collagens from most mammalian species. Rat reactivity reported in scientific literature (PMID: 16774905)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Alternate Names for Collagen IV alpha 1 Antibody [Biotin]

  • alpha-1 chain
  • arresten
  • COL4A1 NC1 domain
  • COL4A1
  • collagen alpha-1(IV) chain
  • Collagen IV alpha 1
  • collagen IV, alpha-1 polypeptide
  • collagen, type IV, alpha 1
  • Collagen-4
  • EC 3.4.23
  • EC 6.3.1.2

Background

These antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Collagen IV alpha 1 Antibody (NB120-6581) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-P.

Reviews using NB120-6581:
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Collagen IV alpha 1 NB120-6581
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reviewed by:
Yves Heremans
IHC-P Mouse 09/21/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Pancreas
SpeciesMouse

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Yves Heremans
09/21/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol COL4A1
Entrez