Collagen IV Antibody [DyLight 755]

• Reactivity: Hu, Mu, Rt, Bv, Fe
• Applications: WB, ELISA, ICC/IF, IHC, IP, IHC, KO, MS
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 755

Collagen IV Antibody [DyLight 755] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Collagen IV from human and bovine placenta (Uniprot: P02462)
• Localization: Secreted and Cell surface (Potential).
• Specificity: Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL4A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockout Validated
  • Super-Resolution Microscopy
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB120-6586. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Rat reactivity reported in scientific literature (PMID: 16774905)

Feline reactivity reported in scientific literature (PMID: 33091431).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Collagen IV Antibody [DyLight 755]

• BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR
• Collagen IV

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL4A1