Collagen III alpha 1/COL3A1 Antibody

Images

 
Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with COL3A1 antibody [C2C3], C-term diluted at 1:5000. ...read more
Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Analysis of paraffin-embedded PAPILLARY CA_STROMAL CELLS, using antibody at 1:250 dilution.
Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - COL3A1 antibody [C2C3], C-term detects COL3A1 protein by Western blot analysis. A. 30 ug Neuro2A whole lysate/extract B. 30 ug NIH-3T3 whole cell ...read more
Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - A. 50 ug rat liver lysate/extract 5% SDS-PAGE COL3A1 antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to ...read more
Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - A. 30 ug PC-12 whole cell extract.B. 30 ug Rat2 whole cell extract. 5 % SDS-PAGE COL3A1 antibody [C2C3], C-term dilution: 1:1000
Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Mouse tissue extracts (30 ug) was separated by 5 % SDS-PAGE, and the membrane was blotted with COL3A1 antibody [C2C3], C-term at a dilution of 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RMSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Collagen III alpha 1/COL3A1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human COL3A1. The exact sequence is proprietary.
Localization
Secreted; extracellular space; extracellular matrix
Predicted Species
Bovine (94%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL3A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
WB: Performed under reducing conditions (lysates boiled with BME).
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-15946 in the following applications:

  • WB
    1 publication

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 28794502). Chicken (83%), Xenopus laevis (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1% BSA, and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Collagen III alpha 1/COL3A1 Antibody

  • alpha1 (III) collagen
  • COL3A1
  • Collagen 3
  • collagen alpha-1(III) chain
  • Collagen III alpha 1
  • collagen, fetal
  • collagen, type III, alpha 1
  • Collagen-3
  • EDS4A
  • Ehlers-Danlos syndrome type IV, autosomal dominant
  • FLJ34534

Background

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen III alpha 1/COL3A1 Antibody (NBP2-15946)(1)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol COL3A1
Entrez