Collagen III alpha 1/COL3A1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Human PAPILLARY CA_STROMAL CELLS, using Collagen III antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Various extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III alpha 1/COL3A1 antibody [C2C3], C-term (NBP2-15946) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Rt, Mu, Rt, Bv, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Collagen III alpha 1/COL3A1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Collagen III alpha 1/COL3A1. The exact sequence is proprietary.
• Localization: Secreted; extracellular space; extracellular matrix
• Predicted Species: Mouse (92%), Rat (92%), Rhesus Macaque (98%), Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL3A1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Frozen Reported in scientific literature (PMID: 32345960)
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Western Blot 1:500 - 1:3000
• Theoretical MW: 139 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 28794502). Chicken (83%), Xenopus laevis (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Collagen III alpha 1/COL3A1 Antibody - BSA Free

• alpha1 (III) collagen
• alpha-1 type III collagen
• COL3A1
• Collagen 3
• collagen alpha-1(III) chain
• Collagen III alpha 1
• collagen, fetal
• collagen, type III, alpha 1
• Collagen-3
• collagenIII
• Collagen-III
• EDS4A
• EDSVASC
• Ehlers-Danlos syndrome type IV, autosomal dominant
• FLJ34534
• PMGEDSV

Background

Collagen III alpha 1, also referred to as collagen type III alpha 1 or COL3A1 for short, was first described in 1971 and is a member of the collagen superfamily and encoded COL3A1 gene (1, 2). In general, collagen III is an extracellular matrix protein that is synthesized as a preprocollagen followed by cleaving of the signal peptide to form the procollagen (1). The human COL3A1 gene is located on chromosome 2q32.2 and collagen III is synthesized as a homotrimer consisting of three identical alpha procollagen chains which are stabilized by disulfide bonds (1,2,3). Each alpha chain is 1466 amino acids (aa) in length with a theoretical molecular weight of 139 kDa for a single alpha chain (1). Structurally, each alpha chain is a left-handed helix which then join together to form a right-handed triple helix (1,2). C-terminal and N-terminal proteinases remove the globular ends of the procollagen to form the type III collagen (1).

Collagen III is a fibrillar collagen that constitutes 5-20% of all collagen in the body (1). It provides structural integrity and is found in many hallow organs and soft connective tissue including the vascular system, skin, lung, uterus, and intestine (1,2). Additionally, collagen III has be found to be associated with type I collagen in the same fibrils (1). Collagen III interacts with signaling integrins to carry out other key functions including cell adhesion, proliferation, and differentiation (1).

Mutations in the COL3A1 gene has been associated with a variety of human diseases, the most well-known being a group of connective tissue disorders termed Ehlers-Danlos Syndromes (1,2,4). Vascular Ehlers-Danlos Syndrome is a specific subtype that is considered the most severe and although the clinical manifestations vary, symptoms include thin skin and fragile blood vessels and can often result in both lung and heart complications (1,4). COL3A1 is also associated with glomerulopathies, or diseases of the glomeruli, which are characterized by an abundance of extracellular matrix (3). Collagenofibrotic glomerulopathy is one specific rare renal disease that is characterized by excessive levels of collagen III (3).

References

1. Kuivaniemi, H., & Tromp, G. (2019). Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. https://doi.org/10.1016/j.gene.2019.05.003

2. Ricard-Blum S. (2011). The collagen family. Cold Spring Harbor perspectives in biology. https://doi.org/10.1101/cshperspect.a004978

3. Cohen A. H. (2012). Collagen Type III Glomerulopathies. Advances in chronic kidney disease. https://doi.org/10.1053/j.ackd.2012.02.017

4. Olson, S. L., Murray, M. L., & Skeik, N. (2019). A Novel Frameshift COL3A1 Variant in Vascular Ehlers-Danlos Syndrome. Annals of vascular surgery. https://doi.org/10.1016/j.avsg.2019.05.057

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen III alpha 1/COL3A1 Antibody (NBP2-15946)(3)

Publications using NBP2-15946

• He X, Cheng R, Huang C, Takahashi Y A novel role of LRP5 in tubulointerstitial fibrosis through activating TGF-beta/Smad signaling Signal Transduction and Targeted Therapy 2020-01-01 [PMID: 32345960] (IHC-Fr, Mouse)
• Gedik N, Kruger M, Thielmann M et al. Proteomics/phosphoproteomics of left ventricular biopsies from patients with surgical coronary revascularization and pigs with coronary occlusion/reperfusion: remote ischemic preconditioning Sci Rep 2017-08-09 [PMID: 28794502] (WB, Porcine)
• Luo J, Liu X, Liu J et al. Activation of TGF-beta1 by AQP3-Mediated H2O2 Transport into Fibroblasts of a Bleomycin-Induced Mouse Model of Scleroderma Journal of Investigative Dermatology 2016-07-22 [PMID: 27456753] (WB, WB, Mouse)

Bioinformatics

• Gene Symbol: COL3A1