Collagen V Antibody (1E2-E4/Col5) - BSA Free Summary
Acid-digested pepsin soluble dog type V collagen
Highly specific for type V collagen. It has been shown to have no cross-reactivity with type I,
III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other
connective tissue proteins (laminin, fibronectin, elastin).
Protein A or G purified
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ELISA: NBP1-05118 can be used for detection of collagens by ELISA. NBP1-05118 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
WB: In immunoblotting NBP1-05118 detects human type V collagen only in its native triple helix form.
IHC: NBP1-05118 has been used succesfully for immunohistology on paraffin embedded (1) and frozen unfixed sections of human (2), bovine (3) and dog (4) skin, on rabbit (1) and foetal bovine cornea (4), and of new dog tissue associated with a biomaterial implant (5). If fixation of tissue is required, acetone or ethanol is recommended.
Alternate Names for Collagen V Antibody (1E2-E4/Col5) - BSA Free
alpha 1 type V collagen
collagen alpha-1(V) chain
collagen, type V, alpha 1
Type V collagen is a minor component of the connective tissue, although it is present in many different types of connective tissue. Patients with defects in the type V collagen (Ehlers-Danlos syndrome) have weakened connective tissue characterized by hyperstrechable joints and fragile, easily bruisable skin.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Collagen V Antibody (NBP1-05118). (Showing 1 - 1 of 1 FAQs).
I can't find the recommended dilutions of NBP1-05118 to do Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Western Blot in your datasheet. Please tell me, thank you.
We recommend starting dilutions of: WB, 1:1000-1:2000 and IHC 1:100-1:200. The antibody should be less diluted in IHC-P than IHC-F as the epitope is harder to find in IHC-P. These are recommended dilutions and you should optimize these in order to get the best result.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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