Collagen V Antibody (1E2-E4/Col5) - BSA Free

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Western Blot: Collagen V Antibody (1E2-E4/Col5) [NBP1-05118] - Staining with 1E2-E4/Col5 on Dog Type I, III, V and Human Type I, III, and V in rows 1-6 respectively. (1. Werkmeister)

Product Details

Summary
Reactivity Hu, Po, Bv, Ca, Ma, Rb, Sh, Ch(-), Gp(-), Mu(-), Rt(-)Species Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clone
1E2-E4/Col5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Collagen V Antibody (1E2-E4/Col5) - BSA Free Summary

Immunogen
Acid-digested pepsin soluble dog type V collagen
Specificity
Highly specific for type V collagen. It has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
COL5A1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
ELISA: NBP1-05118 can be used for detection of collagens by ELISA. NBP1-05118 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well. WB: In immunoblotting NBP1-05118 detects human type V collagen only in its native triple helix form. IHC: NBP1-05118 has been used succesfully for immunohistology on paraffin embedded (1) and frozen unfixed sections of human (2), bovine (3) and dog (4) skin, on rabbit (1) and foetal bovine cornea (4), and of new dog tissue associated with a biomaterial implant (5). If fixation of tissue is required, acetone or ethanol is recommended.
Publications
Read Publications using NBP1-05118.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Collagen V Antibody (1E2-E4/Col5) - BSA Free

  • alpha 1 type V collagen
  • Collagen 5
  • collagen alpha-1(V) chain
  • collagen, type V, alpha 1
  • Collagen-5
  • EC 2.7.7.6
  • EC 6.1.1

Background

Type V collagen is a minor component of the connective tissue, although it is present in many different types of connective tissue. Patients with defects in the type V collagen (Ehlers-Danlos syndrome) have weakened connective tissue characterized by hyperstrechable joints and fragile, easily bruisable skin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Po, Bv, Ca, Ma, Rb, Sh, Ch(-), Gp(-), Mu(-), Rt(-)
Applications: WB, ELISA, IHC, IHC-Fr, IHC-P

Publications for Collagen V Antibody (NBP1-05118)(2)

We have publications tested in 1 confirmed species: Porcine.


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Showing Publications 1 - 2 of 2.
Publications using NBP1-05118 Applications Species
Velez CL. Integrinas y su regulacion por el sistema inmune durante la gestacion porcina. Thesis. 2017 (Porcine) Porcine
Werkmeister J Ramshaw J. Monoclonal antibodies to Type V Collagen for Immunohistological Examination of New Tissue Deposition Associated with Biomaterial Implants. J Histochemistry and Cytochemistry. 1991 [PMID: 1918939]

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Collagen V Antibody (NBP1-05118). (Showing 1 - 1 of 1 FAQs).

  1. I can't find the recommended dilutions of NBP1-05118 to do Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Western Blot in your datasheet. Please tell me, thank you.
    • We recommend starting dilutions of: WB, 1:1000-1:2000 and IHC 1:100-1:200. The antibody should be less diluted in IHC-P than IHC-F as the epitope is harder to find in IHC-P. These are recommended dilutions and you should optimize these in order to get the best result.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COL5A1