Collagen I/II/III Antibody (NLI/22) [Janelia Fluor® 549]

Product Discontinued

Collagen I/II/III Antibody (NLI/22) [Janelia Fluor® 549] Summary

• Immunogen: Human placental collagen type 1
• Specificity: NB100-64521 reacts with collagens type I, II and III. It does not react with human collagen types IV, V or VI, human plasma proteins or albumin from various species.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL1A1
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: IgM purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Collagen I/II/III Antibody (NLI/22) [Janelia Fluor® 549]

• Alpha-1 type I collagen
• collagen alpha 1 chain type I
• collagen alpha-1(I) chain
• collagen of skin, tendon and bone, alpha-1 chain
• collagen, type I, alpha 1
• OI4
• pro-alpha-1 collagen type 1

Background

Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL1A1