CIDEC Antibody - BSA Free

Western Blot: CIDEC Antibody [NB100-430] - Analysis of CIDEC using NB100-430 at 1:1000 dilution on 30ug HepG2 cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CIDEC Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CIDEC Antibody - BSA Free (NB100-430) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-CIDEC Antibody: Cited in 16 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Residues 10-25 LLYPKSLSRHVSVRTS] of the 27 kDa human FSP27 (CIDE-3) protein.
• Localization: Cytoplasmic
• Specificity: This is specific for CIDE-3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CIDEC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:1000
• Application Notes: This antibody may be used for Western blot.Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 23466592) No other assays have been tested. Suggested working dilutions: * Western Blot - 1:500 ~ 1:1,000 *The investigator should determine the optimal working dilution for a specific application. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 26.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CIDEC Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CIDEC Antibody - BSA Free

• cell death activator CIDE-3
• cell death-inducing DFFA-like effector c
• Cell death-inducing DFFA-like effector protein C
• CIDE-3
• CIDEC
• CIDEX
• Fat-specific protein FSP27 homolog
• FLJ20871
• FSP27
• FSP27fat specific protein 27

Background

DNA fragmentation is one of the critical steps in apoptosis, which is induced by DNA fragmentation factor (DFF). DFF is composed of two subunits, a 40 kDa caspase-activated nuclease (DFF40) and a 45 kDa inhibitor (DFF45). Recently a novel family of cell-death-inducing DFF45-like effectors (CIDEs) has been identified. Human CIDE-3 is a novel member of CIDEs. There are 2 transcripts, CIDE-3 and CIDE-3alpha, were present in HepG2 and A375 cells. Consistent with its chromosome localization at 3p25, a region associated with high frequency loss of heterozygosity in many tumors, CIDE-3 may play an important role in prevention of tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CIDEC Antibody (NB100-430)(17)

Publications using NB100-430

• Chhetri A, Park C, Kim H et Al. TMEM135 deficiency improves hepatic steatosis by suppressing CD36 in a SIRT1-dependent manner Mol Metab 2024-12-09 [PMID: 39647810]
• Chiang YP, Li Z, He M et al. Sphingomyelin synthase-related protein SMSr is a phosphatidylethanolamine phospholipase C that promotes nonalcoholic fatty liver disease Journal of Biological Chemistry 2023-08-14 [PMID: 37586586] (Western Blot, Mouse)
• Barsky M, Merkison J, Hosseinzadeh P, et al. Fetal programming of polycystic ovary syndrome: Effects of androgen exposure on prenatal ovarian development The Journal of steroid biochemistry and molecular biology 2021-01-27 [PMID: 33515680] (WB, Mouse)
• Zhang L, Zhang Z, Li C et al. S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis Cell Mol Gastroenterol Hepatol 2020-10-16 [PMID: 33075563] (WB, Mouse)
• Li P, Lu M, Shi J et al. Lung mesenchymal cells elicit lipid storage in neutrophils that fuel breast cancer lung metastasis Nat. Immunol. 2020-09-21 [PMID: 32958928] (WB, Mouse)
• Herrera-Marcos LV, Sancho-Knapik S, Gabas-Rivera C et al. Pgc1a is responsible for the sex differences in hepatic Cidec/Fsp27 beta mRNA expression in hepatic steatosis of mice fed a Western diet Am. J. Physiol. Endocrinol. Metab. 2019-12-17 [PMID: 31846369]
• Zhao L, Zhao J, Gao R, et al. miR-130b is a Potent Stimulator of Hepatic VLDL Assembly and Secretion via Marked Induction of Microsomal Triglyceride Transfer Protein (MTP) Am. J. Physiol. Endocrinol. Metab. 2019-12-10 [PMID: 31821038]
• Huang HJ, Holub C, Rolzin P et al. Adipocyte Hypertrophy and Improved Postprandial Lipid Response in Beta 2 Syntrophin Deficient Mice Cell. Physiol. Biochem. 2019-08-13 [PMID: 30990585] (WB, Mouse)
• Riis S, Christensen B, Nellemann B et al. Molecular adaptations in human subcutaneous adipose tissue after ten weeks of endurance exercise training in healthy males J. Appl. Physiol. 2018-12-20 [PMID: 30571288] (Western Blot, Mouse)
• Sharma VM, Vestergaard ET, Jessen N et al. Growth hormone acts along PPARg-FSP27 axis to stimulate lipolysis in human adipocytes. Am. J. Physiol. Endocrinol. Metab. 2018-10-16 [PMID: 30325658] (WB, Human)
• He J, Zhang B, Gan H. CIDEC Is Involved in LPS-Induced Inflammation and Apoptosis in Renal Tubular Epithelial Cells. Inflammation 2018-06-30 [PMID: 29959627]
• Zhang J, Zamani M et al. AUP1 (Ancient Ubiquitous Protein 1) Is a Key Determinant of Hepatic Very-Low-Density Lipoprotein Assembly and Secretion. Arterioscler Thromb Vasc Biol 2017-01-04 [PMID: 28183703] (WB, Human)
• Hasenfuss SC, Bakiri L, Thomsen MK et al. Regulation of steatohepatitis and PPAR-gamma signaling by distinct AP-1 dimers. Cell Metab. 2014-01-07 [PMID: 24411941] (WB, Mouse)
• Jang HS, Kim JI, Jung KJ et al. Bone marrow-derived cells play a major role in kidney fibrosis via proliferation and differentiation in the infiltrated site. Biochim Biophys Acta 2013-03-02 [PMID: 23466592] (ICC/IF, Mouse)
• Luong QV Determining the Physiological Contribution of Adipocyte Subpopulations Thesis 2019-08-01 (WB, Mouse)
• Swist E, Chen Q, Qiao C, Caldwell D, Gruber H, Scoggan KA. Excess dietary iodine differentially affects thyroid gene expression in diabetes, thyroiditis-prone versus -resistant BioBreeding (BB) rats. Mol Nutr Food Res;55(12):1875-86. 2011-12-01 [PMID: 22058052] (WB, Rat)
• Guillen N, Navarro MA, Arnal C et al. Microarray analysis of hepatic gene expression identifies new genes involved in steatotic liver. Physiol Genomics;37(3):187-198. 2009-01-01 [PMID: 19258494] (WB, Mouse)

Review for CIDEC Antibody (NB100-430) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 19258494
• Species: Mouse

Bioinformatics

• Gene Symbol: CIDEC
• Entrez:
  • 63924(Human)
  • 14311(Mouse)
• Uniprot:
  • Q96AQ7(Human)