DFF40/CAD Antibody (36A349)

Images

 
Western Blot: DFF40 Antibody (36A349) [NB120-13592] - analysis of 30 ug of HeLa cell lysate with this antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
36A349
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

DFF40/CAD Antibody (36A349) Summary

Immunogen
Anti-DFF40 monoclonal antibody was raised against a peptide corresponding to amino acids 205 to 222 of human DFF40.
Specificity
In HeLa, a 40 kDa band is observed.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
DFFB
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Publications
Read Publications using
NB120-13592 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for DFF40/CAD Antibody (36A349)

  • CAD
  • CADCaspase-activated nuclease
  • Caspase-activated deoxyribonuclease
  • Caspase-activated DNase
  • CPAN
  • CPANDNA fragmentation factor 40 kDa subunit
  • DFF2
  • DFF40
  • DFF-40DFF40DFF2DNA fragmentation factor subunit beta
  • DFFB
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
  • EC 3.-

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribo-nuclease) (1,2). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40 (3,4). Human DFF45 (Cat. # IMG-114 & 115) and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (5). Upon cleavage of DFF45/ICAD by activated caspases, DFF40/CAD is released and activated and eventually causes degradation of DNA in the nuclei1 (4). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DFF40/CAD Antibody (NB120-13592)(3)

Reviews for DFF40/CAD Antibody (NB120-13592) (0)

There are no reviews for DFF40/CAD Antibody (NB120-13592). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for DFF40/CAD Antibody (NB120-13592). (Showing 1 - 1 of 1 FAQs).

  1. Does Novus offer any CAD (caspase activated DNase) antibodies that specifically look at the activation state of CAD? And if so, how well does this antibody work with chicken?
    • CAD/DFF40/DFFB is inhibited by being bound by DFFA. Because of this manner of inhibition, antibodies will not be able to recognize the difference between activated and inhibited DFFB, so we do not have a product specifically for activated or inhibited DFFB and this product will probably be nearly impossible to make. Unfortunately, the human and chicken sequence appear to be divergent for any of our immunogens to recommend any antibody to test for cross-reactivity with chicken. I apologize for the inconvenience.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB120-13592AF405
Alexa Fluor 488 Labeled NB120-13592AF488
Alexa Fluor 647 Labeled NB120-13592AF647
Alexa Fluor 700 Labeled NB120-13592AF700
Biotin Labeled NB120-13592B
DyLight 350 Labeled NB120-13592UV
DyLight 405 Labeled NB120-13592V
DyLight 488 Labeled NB120-13592G
DyLight 550 Labeled NB120-13592R
DyLight 650 Labeled NB120-13592C
DyLight 680 Labeled NB120-13592FR
DyLight 755 Labeled NB120-13592IR
HRP Labeled NB120-13592H

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Bioinformatics

Gene Symbol DFFB
Entrez