CIDEA Antibody - BSA Free

Images

 
Western Blot: CIDEA Antibody [NBP1-76950] - Mouse heart tissue lysate with CIDE-A antibody at 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: CIDEA Antibody [NBP1-76950] - Mouse Heart cells with CIDE-A antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: CIDEA Antibody [NBP1-76950] - Analysis of CIDE-A in human brain tissue with CIDE-A antibody at 2.5 ug/mL.
Immunocytochemistry/ Immunofluorescence: CIDEA Antibody [NBP1-76950] - Human brain tissue with CIDE A antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: CIDEA Antibody [NBP1-76950] - Mouse heart tissue with CIDE-A antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

CIDEA Antibody - BSA Free Summary

Immunogen
Antibody was raised against an 18 amino acid peptide near the carboxy terminus of mouse CIDE-A. The immunogen is located within the last 50 amino acids of CIDE-A. Amino Acid Squence: GDTEEQPSPKPSTKG
Specificity
It has no cross activity to CIDE-B.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CIDEA
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
CIDEA Peptide (NBP1-76950PEP)
Reviewed Applications
Read 1 Review rated 3
using
NBP1-76950 in the following applications:

Publications
Read Publications using
NBP1-76950 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for CIDEA Antibody - BSA Free

  • cell death activator CIDE-A
  • cell death-inducing DFFA-like effector aCIDE-A
  • CIDEA

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-A is expressed in many tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CIDEA Antibody (NBP1-76950)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


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ICC/IF
(1)
IHC
(1)
IHC-P
(1)
WB
(4)
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(5)
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Showing Publications 1 - 7 of 7.
Publications using NBP1-76950 Applications Species
Son Y, Choi C, Song C et al. Development of CIDEA reporter mouse model and its application for screening thermogenic drugs Scientific reports Sep 16 2021 [PMID: 34531447] (ICC/IF, Mouse) ICC/IF Mouse
Choi C, Son Y, Kim J Et Al. TM4SF5 knockout protects mice from diet-induced obesity partly by regulating autophagy in adipose tissue Diabetes Jun 29 2021 [PMID: 34187836] (WB) WB
Irungbam K, Roderfeld M, Glimm H et al. Cholestasis impairs hepatic lipid storage via AMPK and CREB signaling in hepatitis B virus surface protein transgenic mice Lab. Invest. Jul 1 2020 [PMID: 32612285] (IHC, Mouse) IHC Mouse
Mohamedi Y, Fontanil T, Cobo T et al. The Differential Expression of Cide Family Members is Associated with Nafld Progression from Steatosis to Steatohepatitis Sci Rep May 16 2019 [PMID: 31097771] (WB, Mouse) WB Mouse
Hu, W;Ru, Z;Zhou, Y;Xiao, W;Sun, R;Zhang, S;Gao, Y;Li, X;Zhang, X;Yang, H; Lung cancer-derived extracellular vesicles induced myotube atrophy and adipocyte lipolysis via the extracellular IL-6-mediated STAT3 pathway Biochim Biophys Acta Mol Cell Biol Lipids Apr 17 2019 [PMID: 31002945] (WB, Mouse) WB Mouse
Rohm M, Schafer M, Laurent V et al. An AMP-activated protein kinase-stabilizing peptide ameliorates adipose tissue wasting in cancer cachexia in mice. Nat. Med. Aug 29 2016 [PMID: 27571348] (WB) WB
Liu H, Wang C, Chang Z Et al. Losartan Attenuates Insulin Resistance and Regulates Browning Phenomenon of White Adipose Tissue in ob/ob Mice Current Issues in Molecular Biology Oct 28 2021 (IHC-P, Mouse) IHC-P Mouse

Review for CIDEA Antibody (NBP1-76950) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-76950:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot CIDEA NBP1-76950
Enlarge
3
reviewed by:
Johana Lambert
WB Mouse 11/16/2018
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Summary

ApplicationWestern Blot
Sample TestedMesenchymal stem cells differentiated into adipocytes
SpeciesMouse
Lot2089-1801

Comments

CommentsDark bands at ~15 and ~37. Difficult to determine which, if either is CIDEA.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CIDEA Antibody (NBP1-76950) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for CIDEA Antibody (NBP1-76950)

Discover related pathways, diseases and genes to CIDEA Antibody (NBP1-76950). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

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1
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Johana Lambert
11/16/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CIDEA
Uniprot