CIDEA Antibody - BSA Free

Western Blot: CIDEA Antibody [NBP1-76950] - Mouse heart tissue lysate with CIDE-A antibody at 1:500 dilution.

Immunohistochemistry: CIDEA Antibody - BSA Free [NBP1-76950] - Immunohistochemistry of CIDEA in mouse heart tissue with CIDEA antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: CIDEA Antibody - BSA Free [NBP1-76950] - Immunofluorescence of CIDEA in Mouse Heart cells with CIDEA antibody at 20 ug/mL.

Immunocytochemistry/ Immunofluorescence: CIDEA Antibody - BSA Free [NBP1-76950] - Immunofluorescence of CIDEA in human brain tissue with CIDE A antibody at 20 ug/mL.Green: CIDEA Antibody Blue: DAPI staining

Immunohistochemistry: CIDEA Antibody - BSA Free [NBP1-76950] - Immunohistochemistry of CIDEA in human brain tissue with CIDE A antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CIDEA Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CIDEA Antibody - BSA Free (NBP1-76950) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-CIDEA Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against an 18 amino acid peptide near the carboxy terminus of mouse CIDE-A. The immunogen is located within the last 50 amino acids of CIDE-A. Amino Acid Squence: GDTEEQPSPKPSTKG
• Specificity: It has no cross activity to CIDE-B.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CIDEA
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin
  • Western Blot 0.5 ug/mL
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  CIDEA Peptide (NBP1-76950PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for CIDEA Antibody - BSA Free

• cell death activator CIDE-A
• cell death-inducing DFFA-like effector aCIDE-A
• CIDEA

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-A is expressed in many tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CIDEA Antibody (NBP1-76950)(11)

Publications using NBP1-76950

• Chan Yoon Park, Donguk Kim, Min Kyeong Seo, Jimin Kim, Han Choe, Jong-Hyeok Kim, Joon Pio Hong, Yeon Ji Lee, Yoonseok Heo, Hwa Jung Kim, Hye Soon Park, Yeon Jin Jang Dysregulation of Lipid Droplet Protein Expression in Adipose Tissues and Association with Metabolic Risk Factors in Adult Females with Obesity and Type 2 Diabetes. The Journal of nutrition 2023-03-21 [PMID: 36931749]
• Liu H, Wang C, Chang Z Et al. Losartan Attenuates Insulin Resistance and Regulates Browning Phenomenon of White Adipose Tissue in ob/ob Mice Curr Issues Mol Biol 2021-12-10 [PMID: 34889901]
• Son Y, Choi C, Song C et al. Development of CIDEA reporter mouse model and its application for screening thermogenic drugs Scientific reports 2021-09-16 [PMID: 34531447] (ICC/IF, Mouse)
• Wang CH, Liu HM, Chang ZY et al. Losartan Prevents Hepatic Steatosis and Macrophage Polarization by Inhibiting HIF-1? in a Murine Model of NAFLD International Journal of Molecular Sciences 2021-07-22 [PMID: 34360607] (Western Blot)
• Choi C, Son Y, Kim J Et Al. TM4SF5 knockout protects mice from diet-induced obesity partly by regulating autophagy in adipose tissue Diabetes 2021-06-29 [PMID: 34187836] (WB)
• Gao YP, Li L, Yan J et al. Down-Regulation of CIDEA Promoted Tumor Growth and Contributed to Cisplatin Resistance by Regulating the JNK-p21/Bad Signaling Pathways in Esophageal Squamous Cell Carcinoma Frontiers in Oncology 2021-02-03 [PMID: 33614508] (Western Blot)
• Irungbam K, Roderfeld M, Glimm H et al. Cholestasis impairs hepatic lipid storage via AMPK and CREB signaling in hepatitis B virus surface protein transgenic mice Lab. Invest. 2020-07-01 [PMID: 32612285] (IF/IHC, Mouse)
• Jeenat Ferdous Urmi, Hiroaki Itoh, Keiko Muramatsu-Kato, Yukiko Kohmura-Kobayashi, Natsuyo Hariya, Divyanu Jain, Naoaki Tamura, Toshiyuki Uchida, Kazunao Suzuki, Yoshihiro Ogawa, Nobuaki Shiraki, Kazuki Mochizuki, Takeo Kubota, Naohiro Kanayama Plasticity of histone modifications around Cidea and Cidec genes with secondary bile in the amelioration of developmentally-programmed hepatic steatosis Scientific Reports 2019-11-19 [PMID: 31745102]
• Mohamedi Y, Fontanil T, Cobo T et al. The Differential Expression of Cide Family Members is Associated with Nafld Progression from Steatosis to Steatohepatitis Sci Rep 2019-05-16 [PMID: 31097771] (WB, Mouse)
• Hu, W;Ru, Z;Zhou, Y;Xiao, W;Sun, R;Zhang, S;Gao, Y;Li, X;Zhang, X;Yang, H; Lung cancer-derived extracellular vesicles induced myotube atrophy and adipocyte lipolysis via the extracellular IL-6-mediated STAT3 pathway Biochim Biophys Acta Mol Cell Biol Lipids 2019-04-17 [PMID: 31002945] (WB, Mouse)
• Rohm M, Schafer M, Laurent V et al. An AMP-activated protein kinase-stabilizing peptide ameliorates adipose tissue wasting in cancer cachexia in mice. Nat. Med. 2016-08-29 [PMID: 27571348] (WB)

Review for CIDEA Antibody (NBP1-76950) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Johana Lambert WB Mouse 11/16/2018

Summary

• Application: Western Blot
• Sample Tested: Mesenchymal stem cells differentiated into adipocytes
• Species: Mouse
• Lot: 2089-1801

Comments

• Comments: Dark bands at ~15 and ~37. Difficult to determine which, if either is CIDEA.

Bioinformatics

• Gene Symbol: CIDEA
• Uniprot:
  • AAC34985(Mouse)