CIDEA Antibody - BSA Free

Western Blot: CIDEA Antibody [NBP1-76951] - Human brain tissue lysate in the absence (A) or presence (B) of peptide (2085P) with CIDE-A antibody at 1:2000 dilution.

Immunocytochemistry/ Immunofluorescence: CIDEA Antibody - BSA Free [NBP1-76951] - Immunofluorescence of CIDEA in Human Brain cells with CIDEA antibody at 20 ug/mL.

Immunohistochemistry: CIDEA Antibody - BSA Free [NBP1-76951] - Immunohistochemistry of CIDEA in human brain tissue with CIDEA antibody at 5 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CIDEA Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 18 amino acid peptide near the carboxy terminus of human CIDE-A. The immunogen is located within the last 50 amino acids of CIDE-A. Amino Acid Squence: DDKEERPSLRSQAKGRFT
• Specificity: It has no cross activity to CIDE-B.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CIDEA
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 2 ug/ml
• Application Notes: An approximately 23 kDa band can be detected in Western Blot.
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  CIDEA Peptide (NBP1-76951PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for CIDEA Antibody - BSA Free

• cell death activator CIDE-A
• cell death-inducing DFFA-like effector aCIDE-A
• CIDEA

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors. Cell death signals are transduced by DD-, DED-, or CARD-containing molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase DFF40/CAD, which is chaperoned and inhibited by DFF45/ICAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is expressed in many tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CIDEA
• Uniprot:
  • AF041378(Human)