CIDEA Antibody

Images

 
Western Blot: CIDEA Antibody [NBP1-76951] - Human brain tissue lysate in the absence (A) or presence (B) of peptide (2085P) with CIDE-A antibody at 1:2000 dilution.
Immunocytochemistry/ Immunofluorescence: CIDEA Antibody [NBP1-76951] - Immunofluorescence of CIDE-A in Human Brain cells with CIDE-A antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: CIDEA Antibody [NBP1-76951] - Human brain tissue with CIDE-A antibody at 5 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CIDEA Antibody Summary

Immunogen
Antibody was raised against a 18 amino acid peptide near the carboxy terminus of human CIDE-A. The immunogen is located within the last 50 amino acids of CIDE-A.
Specificity
It has no cross activity to CIDE-B.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CIDEA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
CIDE-A antibody can be used for detection of CIDE-A by Western blot at 1:1000 to 1:2000 dilution. An approximately 23 kDa band can be detected. It has no cross activity to CIDE-B.
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
CIDEA Peptide (NBP1-76951PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CIDEA Antibody

  • cell death activator CIDE-A
  • cell death-inducing DFFA-like effector aCIDE-A
  • CIDEA

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors. Cell death signals are transduced by DD-, DED-, or CARD-containing molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase DFF40/CAD, which is chaperoned and inhibited by DFF45/ICAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is expressed in many tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CIDEA
Entrez
Uniprot