cIAP-2/HIAP-1 Antibody

Images

 
Western Blot: cIAP-2/HIAP-1 Antibody [NBP1-27972] - HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence: cIAP-2/HIAP-1 Antibody [NBP1-27972] - HIAD1 staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).
Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody [NBP1-27972] - cIAP2 staining in Oral Squamous Cell Carcinoma (OSCC). Image from verified customer review.
Immunohistochemistry: cIAP-2/HIAP-1 Antibody [NBP1-27972] - Analysis of human adenocarcinoma cells and adjacent glands using anti-cIAP2.
Simple Western: cIAP-2/HIAP-1 Antibody [NBP1-27972] - Simple Western lane view shows a specific band for Ciap2 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.5 mg/ml

cIAP-2/HIAP-1 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human cIAP2 (within residues 100-200). [Swiss-Prot# Q13489]
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BIRC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:800-1:3200
  • Immunohistochemistry-Paraffin 1:800-1:3200
Application Notes
This cIAP2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot analysis, where a band can be seen at approx. 64 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
cIAP-2/HIAP-1 Lysate (NBL1-07985)
Control Peptide
cIAP-2/HIAP-1 Protein (NBP1-27972PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-27972 in the following applications:

Publications
Read Publications using
NBP1-27972 in the following applications:

  • WB
    2 publications

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for cIAP-2/HIAP-1 Antibody

  • AIP1
  • API2
  • BIRC3 baculoviral IAP repeat containing 3
  • BIRC3
  • cIAP2
  • c-IAP2
  • cIAP-2
  • HAIP1
  • HIAP1
  • HIAP-1
  • MALT2
  • MIHC
  • RNF49

Background

c-IAP2 (inhibitor of apoptosis protein-2) belongs to IAPs family and besides exerting antiapoptotic effects through its interaction with multiple cytokines, it participates in inflammation, immunity and mitogenic kinase signal transduction, cell proliferation, invasion and metastasis also. With its E3 ubiquitin-protein ligase activity via interaction with several proteins such as RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF1, BCL10 etc., c-IAP2 can regulate NFkB signaling - positive regulation of canonical and negative regulation of non-canonical pathway. c-IAP2 participates in innate immunity via regulation of pattern recognition receptors- TLRs, NLRs and RLRs, and facilitates ubiquitination of RIPK1/CASP8 for preventing spontaneous formation of ripoptosome - a multi-protein complex which kills cancer cells in caspase-dependent/independent pathways. Knockout of c-IAP2 leads to excessive apoptosis, whereas, its overexpression has been found in several malignancies such as lymphoma and ovarian, colon, cervical cancer etc, and c-IAP2 chromosomal aberrations have been linked MALT lymphoma. c-IAP2 expression is low in normal tissues whereas in cancer its expression increases with the tumor grade and is often correlate with drug resistance, tumor recurrence, invasion and metastasis etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for cIAP-2/HIAP-1 Antibody (NBP1-27972)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(2)
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Filter By Species
Human
(2)
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Review for cIAP-2/HIAP-1 Antibody (NBP1-27972) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-27972:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin cIAP-2/HIAP-1 NBP1-27972
Enlarge
reviewed by:
Anonymous
IHC-P Human 09/17/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedlung
SpeciesHuman

Product General Protocols

View specific protocols for cIAP-2/HIAP-1 Antibody (NBP1-27972): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for cIAP-2/HIAP-1 Antibody (NBP1-27972) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-27972AF405
Alexa Fluor 488 Labeled NBP1-27972AF488
Alexa Fluor 647 Labeled NBP1-27972AF647
Alexa Fluor 700 Labeled NBP1-27972AF700
Biotin Labeled NBP1-27972B
DyLight 405 Labeled NBP1-27972V
DyLight 488 Labeled NBP1-27972G
DyLight 550 Labeled NBP1-27972R
DyLight 650 Labeled NBP1-27972C
FITC Labeled NBP1-27972F
HRP Labeled NBP1-27972H

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Bioinformatics Tool for cIAP-2/HIAP-1 Antibody (NBP1-27972)

Discover related pathways, diseases and genes to cIAP-2/HIAP-1 Antibody (NBP1-27972). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for cIAP-2/HIAP-1 Antibody (NBP1-27972)

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Pathways for cIAP-2/HIAP-1 Antibody (NBP1-27972)

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PTMs for cIAP-2/HIAP-1 Antibody (NBP1-27972)

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Research Areas for cIAP-2/HIAP-1 Antibody (NBP1-27972)

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Blogs on cIAP-2/HIAP-1.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
09/17/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol BIRC3
Entrez
Uniprot