cIAP-2/HIAP-1 Antibody - BSA Free

Western Blot: cIAP-2/HIAP-1 Antibody [NBP1-27972] - HeLa whole cell lysate.

Immunocytochemistry/Immunofluorescence: cIAP-2/HIAP-1 Antibody [NBP1-27972] - HIAD1 staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).

Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody [NBP1-27972] - cIAP2 staining in Oral Squamous Cell Carcinoma (OSCC). Image from verified customer review.

Immunohistochemistry: cIAP-2/HIAP-1 Antibody [NBP1-27972] - Analysis of human adenocarcinoma cells and adjacent glands using anti-cIAP2.

Simple Western: cIAP-2/HIAP-1 Antibody [NBP1-27972] - Simple Western lane view shows a specific band for Ciap2 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.5 mg/ml

cIAP-2/HIAP-1 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide made to an internal portion of human cIAP2 (within residues 100-200). [Swiss-Prot# Q13489]
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BIRC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:800-1:3200
  • Immunohistochemistry-Paraffin 1:800-1:3200
  • Simple Western 1:100
  • Western Blot 1 ug/ml
• Application Notes: This cIAP2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot analysis, where a band can be seen at approx. 64 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  cIAP-2/HIAP-1 Overexpression Lysate

• Control Peptide:

  cIAP-2/HIAP-1 Protein (NBP1-27972PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for cIAP-2/HIAP-1 Antibody - BSA Free

• AIP1
• API2
• BIRC3 baculoviral IAP repeat containing 3
• BIRC3
• cIAP2
• c-IAP2
• cIAP-2
• HAIP1
• HIAP1
• HIAP-1
• MALT2
• MIHC
• RNF49

Background

c-IAP2 (inhibitor of apoptosis protein-2) belongs to IAPs family and besides exerting antiapoptotic effects through its interaction with multiple cytokines, it participates in inflammation, immunity and mitogenic kinase signal transduction, cell proliferation, invasion and metastasis also. With its E3 ubiquitin-protein ligase activity via interaction with several proteins such as RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF1, BCL10 etc., c-IAP2 can regulate NFkB signaling - positive regulation of canonical and negative regulation of non-canonical pathway. c-IAP2 participates in innate immunity via regulation of pattern recognition receptors- TLRs, NLRs and RLRs, and facilitates ubiquitination of RIPK1/CASP8 for preventing spontaneous formation of ripoptosome - a multi-protein complex which kills cancer cells in caspase-dependent/independent pathways. Knockout of c-IAP2 leads to excessive apoptosis, whereas, its overexpression has been found in several malignancies such as lymphoma and ovarian, colon, cervical cancer etc, and c-IAP2 chromosomal aberrations have been linked MALT lymphoma. c-IAP2 expression is low in normal tissues whereas in cancer its expression increases with the tumor grade and is often correlate with drug resistance, tumor recurrence, invasion and metastasis etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for cIAP-2/HIAP-1 Antibody (NBP1-27972)(2)

Publications using NBP1-27972

• Kurita S, Mott JL, Cazanave SC et al. Hedgehog Inhibition Promotes a Switch from Type II to Type I Cell Death Receptor Signaling in Cancer Cells. PLoS ONE. 2011-03-31 [PMID: 21483830] (WB, Human)
• Guicciardi ME, Mott JL, Bronk SF et al. Cellular Inhibitor of Apoptosis 1 (cIAP-1) Degradation by Caspase 8 During TNF-related Apoptosis-inducing Ligand (TRAIL)-induced Apoptosis. Exp Cell Res. 2010-10-13 [PMID: 20951133] (WB, Human)

Review for cIAP-2/HIAP-1 Antibody (NBP1-27972) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 09/17/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: lung
• Species: Human

Bioinformatics

• Gene Symbol: BIRC3
• Entrez:
  • 330(Human)
• Uniprot:
  • Q13489()