Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32)

Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of Chymase/CMA1/Mast Cell on different lysates with Rabbit anti-Chymase/CMA1/Mast Cell antibody at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Chymase/CMA1/Mast Cell Chymase antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human tonsil tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Human small intestine tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human skin tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Chymase/CMA1/Mast Cell Chymase antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: JB74-32
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) Summary

• Description: Novus Biologicals Rabbit Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) (NBP2-75441) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Chymase/CMA1/Mast Cell Chymase aa 198-247 / 247. (SwissProt: P23946 Human; SwissProt: P21844 Mouse; SwissProt: P50339 Rat)
• Localization: Secreted, Cytoplasmic granule.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CMA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32)

• Alpha-chymase
• chymase 1 preproprotein transcript E
• chymase 1 preproprotein transcript I
• chymase 1, mast cell
• Chymase
• chymase, heart
• chymase, mast cell
• CMA1
• CYH
• CYM
• EC 3.4.21
• EC 3.4.21.39
• Mast cell protease I
• MCP3P
• Mcpt5
• MCT1
• MGC119890
• MGC119891

Background

Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CMA1