ChemR23/CMKLR1 Antibody (84939) [Unconjugated]

Non-genomic modulation of ChemR23 expression on neutrophils (A,B) ChemR23 expression on human leukocytes was determined by flow cytometry on vehicle and TNF alpha (10 ng/ml; 20 min)-stimulated cells. Representative histograms shown in A. (C) Neutrophils were treated with vehicle, pro-inflammatory (TNF alpha ; fMLF, 1 μM; IL-8, 100 ng/ml) or anti-inflammatory mediators (annexin A1, 10 nM; alpha MSH, 10 nM; C15, 10 pM) followed by staining for ChemR23. ChemR23 expression was also assessed on isolated human neutrophils before and after flow over activated endothelial cells. (D) ChemR23 expression by wild-type but not ChemR23−/− murine neutrophils. (E) Permeabilized human neutrophils were stained with anti-ChemR23 (with goat-anti-mouse Alexa 488 secondary) and wheat germ agglutinin (WGA-Alexa 647) to visualize the cell membrane. Cells were analysed by confocal microscopy. (F) Human neutrophils were stained for ChemR23 and markers of secretory vesicles (CD35), specific granules (CD66b) and azurophil granules (CD63). (G) Neutrophils were loaded with Fura2-AM and calcium flux responses elicited by control (ionomycin), vehicle, ChemR23 inhibitor (CCX2005, 100 nM), C15 (10 pM), scrambled C15 (C15-S; 10 pM) or chemerin (1 nM). Responses are displayed as delta F340/F380. Graphs show mean values±s.e.m. from three to six independent experiments. *PFAB362N.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 84939
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ChemR23/CMKLR1 Antibody (84939) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human ChemR23
  Met1-Leu371
  Accession # NP_004063
• Specificity: Detects human ChemR23. Stains human ChemR23-transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: CMKLR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ChemR23/CMKLR1 Antibody (84939) [Unconjugated]

• CHEMERINR
• chemokine receptor-like 1
• chemokine-like receptor 1
• ChemR23
• cmklr1
• Dez
• Gpcr27
• G-protein coupled receptor ChemR23
• G-protein coupled receptor DEZ
• MGC126105
• MGC126106
• orphan G-protein coupled receptor, Dez

Background

ChemR23 is a chemoattractant receptor expressed on dendritic cells and activated macrophages. ChemR23 binds Chemerin and functions as a coreceptor for SIV and some primary HIV-1 strains.

1. Samson, M. et al. (1998) Eur. J. Immunol. 28:1689.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ChemR23/CMKLR1 Antibody (MAB362)(8)

Publications using MAB362

• Herova M, Schmid M, Gemperle C, Hersberger M ChemR23, the receptor for chemerin and resolvin E1, is expressed and functional on M1 but not on M2 macrophages. J Immunol, 2015-01-30;194(5):2330-7. 2015-01-30 [PMID: 25637017] (Flow Cytometry, Human)
• Zhou J, Liao D, Zhang S, Cheng N, He H, Ye R Chemerin C9 peptide induces receptor internalization through a clathrin-independent pathway. Acta Pharmacol Sin, 2014-03-24;35(5):653-63. 2014-03-24 [PMID: 24658352] (Flow Cytometry, Human)
• Jenna L Cash, Stefania Bena, Sarah E Headland, Simon McArthur, Vincenzo Brancaleone, Mauro Perretti Chemerin15 inhibits neutrophil‐mediated vascular inflammation and myocardial ischemia‐reperfusion injury through ChemR23 EMBO reports 11/1/2013 [PMID: 23999103]
• Cédric de Poorter, Kevin Baertsoen, Vincent Lannoy, Marc Parmentier, Jean-Yves Springael Consequences of ChemR23 Heteromerization with the Chemokine Receptors CXCR4 and CCR7 PLoS ONE 2/28/2013 [PMID: 23469143]
• Gao L, Faibish D, Fredman G, Herrera B, Chiang N, Serhan C, Van Dyke T, Gyurko R Resolvin E1 and chemokine-like receptor 1 mediate bone preservation. J Immunol, 2012-12-14;190(2):689-94. 2012-12-14 [PMID: 23241890] (Flow Cytometry, ICC, Mouse)
• Vivian Berg, Baldur Sveinbjörnsson, Signy Bendiksen, Jan Brox, Khaled Meknas, Yngve Figenschau Human articular chondrocytes express ChemR23 and chemerin; ChemR23 promotes inflammatory signalling upon binding the ligand chemerin(21-157) Arthritis Research & Therapy 12/30/2010 [PMID: 21192818]
• Skrzeczynska-Moncznik J, Wawro K, Stefanska A, Oleszycka E, Kulig P, Zabel BA, Sulkowski M, Kapinska-Mrowiecka M, Czubak-Macugowska M, Butcher EC, Cichy J Potential role of chemerin in recruitment of plasmacytoid dendritic cells to diseased skin. Biochem. Biophys. Res. Commun., 2009-01-23;380(2):323-7. 2009-01-23 [PMID: 19168032] (Flow Cytometry, Human)
• Martensson UE, Fenyo EM, Olde B, Owman C Characterization of the human chemerin receptor--ChemR23/CMKLR1--as co-receptor for human and simian immunodeficiency virus infection, and identification of virus-binding receptor domains. Virology, 2006-08-10;355(1):6-17. 2006-08-10 [PMID: 16904155] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CMKLR1
• Uniprot:
  • NP_004063()