Leukotriene B4 R1 Antibody

Western Blot: Leukotriene B4 R1 Antibody [NBP2-27422] - Analysis using BLT1 antibody. Lysate from human muscle in the 1) absence, 2) presence of immunizing peptide probed with BLT1 antibody at 3 ug/ml.

Immunohistochemistry-Paraffin: Leukotriene B4 R1 Antibody [NBP2-27422] - Analysis of human liver using BLT1 antibody at 10 ug/ml.

• Reactivity: Hu, Pm, Pm
• Applications: WB, Flow, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Leukotriene B4 R1 Antibody Summary

• Description: Novus Biologicals Rabbit Leukotriene B4 R1 Antibody (NBP2-27422) is a polyclonal antibody validated for use in IHC, WB and Flow. Anti-Leukotriene B4 R1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 1-60 from human BLT1 was used as the immunogen for this antibody (1st extracellular domain).
• Predicted Species: Chimpanzee (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LTB4R
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry reported in scientific literature (PMID 28763622)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10ug/ml~
  • Western Blot 3-5ug/ml~

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in White-cheeked Gibbon, and 83% in orangutan.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for Leukotriene B4 R1 Antibody

• BLT1
• chemokine receptor-like 1
• CMKRL1
• Leukotriene B4 R1
• leukotriene B4 receptor
• Leukotriene B4R1
• LTB4R
• LTB4R1
• LTBR1
• P2Y purinoceptor 7
• purinergic receptor P2Y, G-protein coupled, 7

Background

BLTR2, also known as Leukotriene B4 Receptor 1, is a Chemoattractant Receptor that causes LTB4-induced chemotaxis, calcium mobilization, and inhibition of adenylyl cyclase. Portions of the coding regions of BLTR2 have been duplicated and are part of the 5' UTR noncoding regions of the clusters LS190955 (BLT1) and LS54843 (CIDE-B) on chromosome 14. BLTR2 has been reported in humans in peripheral blood leukocytes, mononuclear lymphocytes, and spleen. ESTs have been isolated from a broad array of human libraries, including brain, eye, fetal lung/testis/B-cell, heart, kidney, melanocyte/uterus/fetal heart, testis, tonsil, and uterus libraries

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Leukotriene B4 R1 Antibody (NBP2-27422)(1)

Publication using NBP2-27422

• Chen X, Stout S, Mueller U et al. Label-Free, LC-MS-Based Assays to Quantitate Small-Molecule Antagonist Binding to the Mammalian BLT1 Receptor SLAS Discov 2017-08-01 [PMID: 28763622] (FLOW, Human)

Bioinformatics

• Gene Symbol: LTB4R
• Uniprot:
  • Q15722(Human)