CCRL2/LCCR Antibody (498321)

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCRL2/L‑CCR Monoclonal Antibody (Catalog # MAB5519, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

CCRL2/L‑CCR/CRAM‑A/B was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse CCRL2/L‑CCR/CRAM‑A/B Monoclonal Antibody (Catalog # MAB5519) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 498321
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCRL2/LCCR Antibody (498321) Summary

• Immunogen: RBL-1 rat basophilic leukemia cell line transfected with mouse CCRL2/LCCR
  Accession # O35457
• Specificity: Detects mouse CCRL2/LCCR in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: Ccrl2
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCRL2/LCCR Antibody (498321)

• CCRL2/LCCR
• LCCR

Background

Lipopolysaccharide-inducible CC chemokine receptor (LCCR), also known as CCRL2, is a 7TM  chemokine receptor-like protein that is expressed on macrophages, glial cells, and mast cells at inflammatory sites. LCCR functions as a receptor for Chemerin, CCL2, 5, 7, and 8. Mouse LCCR shares 49% and 70% aa sequence identity with human and rat LCCR, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Ccrl2
• Uniprot:
  • O35457()