CHAF1A Antibody

Western Blot: CHAF1A Antibody [NB100-74609] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Caf1p150 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: CHAF1A Antibody [NB100-74609] - Human lung cancer. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Western Blot: CHAF1A Antibody [NB100-74609] - Detection of Human Caf1p150 on HeLa whole cell lysate using NB100-74609. Caf1p150 was also immunoprecipitated by rabbit anti-Caf1p150 antibodies NB100-74608 and NB100-74610.

Immunoprecipitation: CHAF1A Antibody [NB100-74609] - Detection of human Caf1p150 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Caf1p150 antibody NB100-74609 used for IP at 3 ug per reaction. Caf1p150 was also immunoprecipitated by another rabbit anti-Caf1p150 antibody. For blotting immunoprecipitated Caf1p150, NB100-74609 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CHAF1A Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 500 and 550 of human chromatin assembly factor 1, subunit A (p150) using the numbering given in entry AAA76736.1 (GeneID 10036).
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHAF1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Control:

  CHAF1A Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CHAF1A Antibody

• CAF
• CAF-1 subunit A
• CAF1
• CAF-1
• CAF1B
• CAF1P150CAF-I 150 kDa subunit
• CAF-I p150
• chromatin assembly factor 1 subunit A
• chromatin assembly factor 1, subunit A (p150)
• chromatin assembly factor I (150 kDa)
• Chromatin assembly factor I p150 subunit
• hp150
• MGC71229
• P150

Background

Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHAF1A
• Entrez:
  • 10036(Human)
• Uniprot:
  • AAA76736.1(Human)