CENPF Antibody


Western Blot: CENPF Antibody [NB500-101] - 25ug of asynchronous and mitotically blocked HeLa lysates were loaded in each respective lane.
Immunocytochemistry/ Immunofluorescence: CENPF Antibody [NB500-101] - Confocal immunofluorescent analysis of HeLa cells using CENPF antibody (NB500-101, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as ...read more
Flow Cytometry: CENPF Antibody [NB500-101] - Detection of CENPF in Hela cells using NB500-101 at a dilution of 1:50 in fixed cells.
Immunocytochemistry/ Immunofluorescence: CENPF Antibody [NB500-101] - CENPF antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Reactivity Hu, BvSpecies Glossary
Applications WB, Flow, ICC/IF, IP
2.5 mg/ml

CENPF Antibody Summary

A bacterial fusion protein from the C-terminus of human CENPF. [UniProt# P49454]
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS with 50% glycerol
0.05% Sodium Azide
2.5 mg/ml
Immunogen affinity purified


Application Notes
This CENPF antibody is useful for Flow Cytometry, Immunoprecipitation, Immunofluorescence/Immunocytochemistry, and Western Blot where a band can be seen at ~330 kDa. In ICC/IF, nuclear staining was observed in HeLa cells.
Reviewed Applications
Read 2 Reviews rated 5
NB500-101 in the following application:

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NB500-101 in the following applications:

Alternate Names for CENPF Antibody

  • AH antigen
  • cell-cycle-dependent 350K nuclear protein
  • CENF
  • CENP-F kinetochore protein
  • CENP-F
  • centromere protein F (350/400kD, mitosin)
  • centromere protein F
  • centromere protein F, 350/400ka (mitosin)
  • centromere protein F, 350/400kDa (mitosin)
  • hcp-1
  • Kinetochore protein CENPF
  • mitosin
  • PRO1779


CENPF interacts with the centromere-kinetochore complex. CENP-F also shows a cell-cycle distribution that is both temporally regulated and diverse in terms of the structural components it localizes to and therefore may play a role in several mitotic events.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CENPF Antibody (NB500-101)(43)

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Equine.

We have publications tested in 5 applications: ICC/IF, IHC-Fr, IHC-P, IHC-WhMt, WB.

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Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using NB500-101 Applications Species
Magidson V, He J, Ault JG et al. Unattached kinetochores rather than intrakinetochore tension arrest mitosis in taxol-treated cells J. Cell Biol. Feb 1 2016 12:00AM [PMID:26833787] (ICC/IF, Human) ICC/IF Human
Zhu L, Wang Z, Wang W et al. Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis. J Biol Chem. [PMID:26378239] (ICC/IF, Human) ICC/IF Human
Kanfer G, Courtheoux T1, Peterka M et al. Mitotic redistribution of the mitochondrial network by Miro and Cenp-F. Nat Commun. [PMID:26259702] (ICC/IF, Human) ICC/IF Human
Guo Y, Zheng Y. Lamins position the nuclear pores and centrosomes by modulating dynein. Mol Biol Cell. [PMID:26246603] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Kaida A, Miura M. Unusual prolongation of radiation-induced G2 arrest in tumor xenografts derived from HeLa cells. Cancer Sci. [PMID:26195156] (IHC-Fr, Human) IHC-Fr Human
Nishikawa R, Goto Y, Kurozumi A et al. MicroRNA-205 inhibits cancer cell migration and invasion via modulation of centromere protein F regulating pathways in prostate cancer. Int J Urol. [PMID:26059417] (WB, IHC-P, Human) WB, IHC-P Human
Bee L, Nasca A, Zanolini A et al. A nonsense mutation of human XRCC4 is associated with adult-onset progressive encephalocardiomyopathy. EMBO Mol Med. [PMID:25872942] (ICC/IF, Human) ICC/IF Human
Nakajima NI, Hagiwara Y, Oike T et al. Pre-exposure to ionizing radiation stimulates DNA double strand break end resection, promoting the use of homologous recombination repair. PLoS One. [PMID:25826455] (ICC/IF, WB, Human) ICC/IF, WB Human
Lee IJ, Lee CW, Lee JH. CaMKKbeta-AMPKalpha2 signaling contributes to mitotic Golgi fragmentation and the G2/M transition in mammalian cells. Cell Cycle. Jul 7 1905 12:00AM [PMID:25590814] (ICC/IF, Human) ICC/IF Human
Mannino M, Gomez-Roman N, Hochegger H, Chalmers AJ. Differential sensitivity of Glioma stem cells to Aurora kinase A inhibitors: implications for stem cell mitosis and centrosome dynamics. Stem Cell Res. [PMID:24879067] (ICC/IF, Human) ICC/IF Human
Show All 43 Publications.

Reviews for CENPF Antibody (NB500-101) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1 application: ICC.

Reviews using NB500-101:
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Images Ratings Applications Species Date Details
 CENPF NB500-101
reviewed by:
Human 03/24/2015


FileView PDF
Immunocytochemistry CENPF NB500-101
reviewed by:
ICC Human 06/05/2014


Special ApplicationsConfocal immunofluorescent analysis of HeLa cells using CENPF antibody (NB500-101, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
FileView PDF

Product General Protocols

View specific protocols for CENPF Antibody (NB500-101): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CENPF Antibody (NB500-101). (Showing 1 - 2 of 2 FAQs).

  1. What residues of human CENP-F was NB500-101 generated against?
    • The exact immunogen sequence is proprietary and cannot be released.
  2. Could you please check whether you can provide which region/sequence this antibody is raised against in the C-terminus of CENPF?
    • Unfortunately, I am not able to provide sequence information further than what we have published. I apologize for the inconvenience.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB500-101AF405
Alexa Fluor (R) 488 Labeled NB500-101AF488
Alexa Fluor (R) 647 Labeled NB500-101AF647
Alexa Fluor (R) 700 Labeled NB500-101AF700
Biotin Labeled NB500-101B
DyLight 405 Labeled NB500-101V
DyLight 488 Labeled NB500-101G
DyLight 550 Labeled NB500-101R
DyLight 650 Labeled NB500-101C
FITC Labeled NB500-101F
HRP Labeled NB500-101H
PE Labeled NB500-101PE

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Blogs on CENPF.

CENPF: At the Center-o'-mere Mitotic Division (Infographic)
Centromere protein F (CENPF) also known as Mitosin, AH antigen, and kinetochore protein CENPF, is a protein that associates with the centromere-kinetochore complex. CENPF forms both a homodimer and a heterodimer. CENPF can be found in different cellul...  Read full blog post.

CENPF Antibodies as Potential Cancer Markers
Centromere protein F (CENPF), also named mitosin, is a large human protein of 3113 amino acid residues. Its expression and localization are cell cycle-dependent. The protein levels are low in G1 phase but elevated from S to early M phase. CENPF is a n...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol CENPF

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