CENPF Antibody - BSA Free

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Biological Strategies: Knockdown Validated: CENPF Antibody [NB500-101] - Kinetochore localization of RZZ and MAD1 are independent of CENP-E and CENP-F. CENP-F depletion does not interfere with the recruitment of ...read more
Biological Strategies: Western Blot: CENPF Antibody [NB500-101] - 25ug of asynchronous and mitotically blocked HeLa lysates were loaded in each respective lane.
Immunocytochemistry/ Immunofluorescence: CENPF Antibody [NB500-101] - Distribution of CENP-F (red), largely associated with the mitotic spindle and a clearly distinguishable frac.
Immunocytochemistry/ Immunofluorescence: CENPF Antibody [NB500-101] - CENPF antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunocytochemistry/ Immunofluorescence: CENPF Antibody [NB500-101] - Confocal immunofluorescent analysis of HeLa cells using CENPF antibody (NB500-101, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, EqSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-WhMt, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

CENPF Antibody - BSA Free Summary

Immunogen
A bacterial fusion protein from the C-terminus of human CENPF. [UniProt# P49454]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CENPF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000immunoprecipitation 1:50
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:50
  • Knockdown Validated
  • Western Blot 1:1500-1:2500
Application Notes
This CENPF antibody is useful for Flow Cytometry, Immunoprecipitation, Immunofluorescence/Immunocytochemistry, and Western Blot (band can be seen at ~330 kDa). In ICC/IF, nuclear staining was observed in HeLa cells. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 20398247). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26195156). Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 24876181).
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB500-101 in the following applications:

Publications
Read Publications using
NB500-101 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CENPF Antibody - BSA Free

  • AH antigen
  • cell-cycle-dependent 350K nuclear protein
  • CENF
  • CENP-F kinetochore protein
  • CENP-F
  • centromere protein F (350/400kD, mitosin)
  • centromere protein F
  • centromere protein F, 350/400ka (mitosin)
  • centromere protein F, 350/400kDa (mitosin)
  • hcp-1
  • Kinetochore protein CENPF
  • mitosin
  • PRO1779

Background

CENPF interacts with the centromere-kinetochore complex. CENP-F also shows a cell-cycle distribution that is both temporally regulated and diverse in terms of the structural components it localizes to and therefore may play a role in several mitotic events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Bv, Eq
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-WhMt, KD

Publications for CENPF Antibody (NB500-101)(51)

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Equine.

We have publications tested in 5 applications: ICC/IF, IHC-Fr, IHC-P, IHC-WhMt, WB.


Filter By Application
ICC/IF
(32)
IHC-Fr
(1)
IHC-P
(3)
IHC-WhMt
(1)
WB
(12)
All Applications
Filter By Species
Human
(35)
Mouse
(2)
Bovine
(1)
Equine
(1)
All Species
Showing Publications 1 - 10 of 51. Show All 51 Publications.
Publications using NB500-101 Applications Species
Becker LCA. Influence of repair proteins and chromatin modifiers on mobility of DNA double-strand breaks induced by heavy ion irradiation. Thesis. 2014 May 16 (ICC/IF, Human) ICC/IF Human
Renda F, Magidson V, Tikhonenko I et al. Effects of malleable kinetochore morphology on measurements of intrakinetochore tension Open Biol Jul 1 2020 [PMID: 32634373] (ICC/IF, Human) ICC/IF Human
Rodriguez-Rodriguez J, Lewis C, Mckinley k et al. Distinct Roles of RZZ and Bub1-kNL1 in Mitotic Checkpoint Signaling and kinetochore Expansion. Current Biology. Oct 1 2018 [PMID: 30415700] (ICC/IF, Human) ICC/IF Human
Sikirzhytski V, Renda F, Tikhonenko I et al. Microtubules assemble near most kinetochores during early prometaphase in human cells J. Cell Biol. Jun 15 2018 [PMID: 29907657] (ICC/IF, Human) ICC/IF Human
Ciossani G, Overlack K, Petrovic A et al. The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases J. Biol. Chem. May 10 2018 [PMID: 29748388] (WB) WB
Chong T, Sarac A, Yao CQ et al. Deregulation of the spindle assembly checkpoint is associated with paclitaxel resistance in ovarian cancer. J Ovarian Res Apr 4 2018 [PMID: 29618387] (WB, Human) WB Human
Yamauchi Motohiro, Shibata Atsushi, Suzuki Keiji et al. Regulation of pairing between broken DNA-containing chromatin regions by Ku80, DNA-PKcs, ATM, and 53BP1. Sci Rep 2017 [PMID: 28155885] (ICC/IF, Human) ICC/IF Human
Magidson V, He J, Ault JG et al. Unattached kinetochores rather than intrakinetochore tension arrest mitosis in taxol-treated cells J. Cell Biol. Feb 1 2016 [PMID: 26833787] (ICC/IF, Human) ICC/IF Human
Zhu L, Wang Z, Wang W et al. Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis. J Biol Chem. [PMID: 26378239] (ICC/IF, Human) ICC/IF Human
Kanfer G, Courtheoux T1, Peterka M et al. Mitotic redistribution of the mitochondrial network by Miro and Cenp-F. Nat Commun. [PMID: 26259702] (ICC/IF, Human) ICC/IF Human
Show All 51 Publications.

Reviews for CENPF Antibody (NB500-101) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB500-101:
Filter by Applications
Immunofluorescence
(1)
Immunocytochemistry
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence CENPF NB500-101
Enlarge
5
reviewed by:
Verified Customer
Immunofluorescence Human 03/24/2015
View

Summary

ApplicationImmunofluorescence
SpeciesHuman
Immunocytochemistry CENPF NB500-101
Enlarge
5
reviewed by:
Verified Customer
Immunocytochemistry Human 06/05/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman

Product General Protocols

View specific protocols for CENPF Antibody (NB500-101): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CENPF Antibody (NB500-101). (Showing 1 - 2 of 2 FAQs).

  1. What residues of human CENP-F was NB500-101 generated against?
    • The exact immunogen sequence is proprietary and cannot be released.
  2. Could you please check whether you can provide which region/sequence this antibody is raised against in the C-terminus of CENPF?
    • Unfortunately, I am not able to provide sequence information further than what we have published. I apologize for the inconvenience.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CENPF Antibody (NB500-101)

Discover related pathways, diseases and genes to CENPF Antibody (NB500-101). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CENPF Antibody (NB500-101)

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Pathways for CENPF Antibody (NB500-101)

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PTMs for CENPF Antibody (NB500-101)

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Research Areas for CENPF Antibody (NB500-101)

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Blogs on CENPF.

CENPF: At the Center-o'-mere Mitotic Division (Infographic)
Centromere protein F (CENPF) also known as Mitosin, AH antigen, and kinetochore protein CENPF, is a protein that associates with the centromere-kinetochore complex. CENPF forms both a homodimer and a heterodimer. CENPF can be found in different cellul...  Read full blog post.

CENPF Antibodies as Potential Cancer Markers
Centromere protein F (CENPF), also named mitosin, is a large human protein of 3113 amino acid residues. Its expression and localization are cell cycle-dependent. The protein levels are low in G1 phase but elevated from S to early M phase. CENPF is a n...  Read full blog post.

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Recent Reviews

5
5
2
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2
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1
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Verified Customer
03/24/2015
Application: Immunofluorescence
Species: Human

Verified Customer
06/05/2014
Application: Immunocytochemistry
Species: Human

Bioinformatics

Gene Symbol CENPF