CENPF Antibody

Images

 
Western Blot: CENPF Antibody [NB500-101] - 25ug of asynchronous and mitotically blocked HeLa lysates were loaded in each respective lane.
Immunocytochemistry/ Immunofluorescence: CENPF Antibody [NB500-101] - Confocal immunofluorescent analysis of HeLa cells using CENPF antibody (NB500-101, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as ...read more
Immunocytochemistry/ Immunofluorescence: CENPF Antibody [NB500-101] - CENPF antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, Flow, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2.5 mg/ml

CENPF Antibody Summary

Immunogen
A bacterial fusion protein from the C-terminus of human CENPF. [UniProt# P49454]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CENPF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1500-1:2500
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunoprecipitation 1:50
Application Notes
This CENPF antibody is useful for Flow Cytometry, Immunoprecipitation, Immunofluorescence/Immunocytochemistry, and Western Blot where a band can be seen at ~330 kDa. In ICC/IF, nuclear staining was observed in HeLa cells.
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 2 Reviews rated 5
using
NB500-101 in the following applications:

Publications
Read Publications using
NB500-101 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
2.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CENPF Antibody

  • AH antigen
  • cell-cycle-dependent 350K nuclear protein
  • CENF
  • CENP-F kinetochore protein
  • CENP-F
  • centromere protein F (350/400kD, mitosin)
  • centromere protein F
  • centromere protein F, 350/400ka (mitosin)
  • centromere protein F, 350/400kDa (mitosin)
  • hcp-1
  • Kinetochore protein CENPF
  • mitosin
  • PRO1779

Background

CENPF interacts with the centromere-kinetochore complex. CENP-F also shows a cell-cycle distribution that is both temporally regulated and diverse in terms of the structural components it localizes to and therefore may play a role in several mitotic events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-82875
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB500-106
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NB100-56598
Species: Hu, Mu
Applications: WB, IHC, IHC-Fr, IHC-P
AF6000
Species: Hu, Mu
Applications: WB, Simple Western, IHC
NB100-353
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IP, CyTOF-ready
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
H00000699-D01P
Species: Hu, Mu
Applications: WB, ICC/IF, PLA
H00051053-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-01086
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-87769
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88769
Species: Hu, Mu
Applications: WB, IHC, IHC-P
H00000468-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NB100-1844
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-88089
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-06603
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC
NBP1-32775
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
NB500-101
Species: Hu, Bv
Applications: WB, Flow, ICC/IF, IP

Publications for CENPF Antibody (NB500-101)(45)

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Equine.

We have publications tested in 5 applications: ICC/IF, IHC-Fr, IHC-P, IHC-WhMt, WB.


Filter By Application
ICC/IF
(28)
IHC-Fr
(1)
IHC-P
(3)
IHC-WhMt
(1)
WB
(10)
All Applications
Filter By Species
Human
(30)
Mouse
(2)
Bovine
(1)
Equine
(1)
All Species
Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using NB500-101 Applications Species
Magidson V, He J, Ault JG et al. Unattached kinetochores rather than intrakinetochore tension arrest mitosis in taxol-treated cells J. Cell Biol. Feb 1 2016 [PMID: 26833787] (ICC/IF, Human) ICC/IF Human
Zhu L, Wang Z, Wang W et al. Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis. J Biol Chem. [PMID: 26378239] (ICC/IF, Human) ICC/IF Human
Kanfer G, Courtheoux T1, Peterka M et al. Mitotic redistribution of the mitochondrial network by Miro and Cenp-F. Nat Commun. [PMID: 26259702] (ICC/IF, Human) ICC/IF Human
Magidson V, Paul R, Yang N et al. Adaptive changes in the kinetochore architecture facilitate proper spindle assembly. Nat Cell Biol 2015 Sep [PMID: 26258631] (ICC/IF) ICC/IF
Guo Y, Zheng Y. Lamins position the nuclear pores and centrosomes by modulating dynein. Mol Biol Cell. [PMID: 26246603] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Kaida A, Miura M. Unusual prolongation of radiation-induced G2 arrest in tumor xenografts derived from HeLa cells. Cancer Sci. [PMID: 26195156] (IHC-Fr, Human) IHC-Fr Human
Nishikawa R, Goto Y, Kurozumi A et al. MicroRNA-205 inhibits cancer cell migration and invasion via modulation of centromere protein F regulating pathways in prostate cancer. Int J Urol. [PMID: 26059417] (WB, IHC-P, Human) WB, IHC-P Human
Lee A, Lyua SK, Kwona H. BAF53 is required for mitotic progression Animal Cells and Systems (ICC/IF, WB, Human) ICC/IF, WB Human
Bee L, Nasca A, Zanolini A et al. A nonsense mutation of human XRCC4 is associated with adult-onset progressive encephalocardiomyopathy. EMBO Mol Med. [PMID: 25872942] (ICC/IF, Human) ICC/IF Human
Nakajima NI, Hagiwara Y, Oike T et al. Pre-exposure to ionizing radiation stimulates DNA double strand break end resection, promoting the use of homologous recombination repair. PLoS One. [PMID: 25826455] (ICC/IF, WB, Human) ICC/IF, WB Human
Show All 45 Publications.

Reviews for CENPF Antibody (NB500-101) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB500-101:
Filter by Applications
IF
(1)
ICC
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence CENPF NB500-101
Enlarge
reviewed by:
Patrizia Lavia
IF Human 03/24/2015
View

Summary

ApplicationImmunofluorescence
SpeciesHuman
FileView PDF
Immunocytochemistry CENPF NB500-101
Enlarge
reviewed by:
Anony- mous
ICC Human 06/05/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsConfocal immunofluorescent analysis of HeLa cells using CENPF antibody (NB500-101, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
FileView PDF

Product General Protocols

View specific protocols for CENPF Antibody (NB500-101): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CENPF Antibody (NB500-101). (Showing 1 - 2 of 2 FAQs).

  1. What residues of human CENP-F was NB500-101 generated against?
    • The exact immunogen sequence is proprietary and cannot be released.
  2. Could you please check whether you can provide which region/sequence this antibody is raised against in the C-terminus of CENPF?
    • Unfortunately, I am not able to provide sequence information further than what we have published. I apologize for the inconvenience.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-101AF405
Alexa Fluor 488 Labeled NB500-101AF488
Alexa Fluor 647 Labeled NB500-101AF647
Alexa Fluor 700 Labeled NB500-101AF700
Biotin Labeled NB500-101B
DyLight 405 Labeled NB500-101V
DyLight 488 Labeled NB500-101G
DyLight 550 Labeled NB500-101R
DyLight 650 Labeled NB500-101C
FITC Labeled NB500-101F
HRP Labeled NB500-101H
PE Labeled NB500-101PE

Additional CENPF Products

Bioinformatics Tool for CENPF Antibody (NB500-101)

Discover related pathways, diseases and genes to CENPF Antibody (NB500-101). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CENPF Antibody (NB500-101)

Discover more about diseases related to CENPF Antibody (NB500-101).
 

Pathways for CENPF Antibody (NB500-101)

View related products by pathway.

PTMs for CENPF Antibody (NB500-101)

Learn more about PTMs related to CENPF Antibody (NB500-101).
 

Research Areas for CENPF Antibody (NB500-101)

Find related products by research area.

Blogs on CENPF.

CENPF: At the Center-o'-mere Mitotic Division (Infographic)
Centromere protein F (CENPF) also known as Mitosin, AH antigen, and kinetochore protein CENPF, is a protein that associates with the centromere-kinetochore complex. CENPF forms both a homodimer and a heterodimer. CENPF can be found in different cellul...  Read full blog post.

CENPF Antibodies as Potential Cancer Markers
Centromere protein F (CENPF), also named mitosin, is a large human protein of 3113 amino acid residues. Its expression and localization are cell cycle-dependent. The protein levels are low in G1 phase but elevated from S to early M phase. CENPF is a n...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Patrizia Lavia
03/24/2015
Application: IF
Species: Human

Anony- mous
06/05/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol CENPF