CENPF Antibody [DyLight 650]


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Product Details

Reactivity Hu, BvSpecies Glossary
Applications WB, Flow, ICC/IF
DyLight 650

CENPF Antibody [DyLight 650] Summary

A bacterial fusion protein from the C-terminus of human CENPF. [UniProt# P49454]
Immunogen affinity purified
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  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
This CENPF antibody is useful for Flow Cytometry, Immunoprecipitation, Immunofluorescence/Immunocytochemistry, and Western Blot where a band can be seen at ~330 kDa. In ICC/IF, nuclear staining was observed in HeLa cells.

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Immunogen affinity purified


Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CENPF Antibody [DyLight 650]

  • AH antigen
  • cell-cycle-dependent 350K nuclear protein
  • CENF
  • CENP-F kinetochore protein
  • CENP-F
  • centromere protein F (350/400kD, mitosin)
  • centromere protein F
  • centromere protein F, 350/400ka (mitosin)
  • centromere protein F, 350/400kDa (mitosin)
  • hcp-1
  • Kinetochore protein CENPF
  • mitosin
  • PRO1779


The kinetophore is a macromolecular structure that is associated with the centromeres of chromosomes and is responsible for establishing and maintaining the connections with the microtubules of the mitotic spindle. The proteins that reside within the chromosomal domain fall into two broad classes: those that associate with the centromere throughout the cell cycle and those that are found at the centromere in a cell cycle specific manner. Members of the first class include CENP-A, CENP-B, and CENP-C. The members of the second class include several ubiquitous cellular proteins such as calmodulin, tubulin, dynein and p34 cdc2 and also CENP-E and CENP-F. CENP-F appears to be the earliest member to interact with the centromere-kinetochore complex. CENP-F also shows a cell-cycle distribution that is both temporally regulated and diverse in terms of the structural components it localizes to and therefore may play a role in several mitotic events. (1-3)


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin Labeled NB500-101B
DyLight 488 Labeled NB500-101G
DyLight 550 Labeled NB500-101R
HRP Labeled NB500-101H

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Bioinformatics Tool for CENPF Antibody (NB500-101C)

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Research Areas for CENPF Antibody (NB500-101C)

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Blogs on CENPF.

CENPF: At the Center-o'-mere Mitotic Division (Infographic)
Centromere protein F (CENPF) also known as Mitosin, AH antigen, and kinetochore protein CENPF, is a protein that associates with the centromere-kinetochore complex. CENPF forms both a homodimer and a heterodimer. CENPF can be found in different cellul...  Read full blog post.

CENPF Antibodies as Potential Cancer Markers
Centromere protein F (CENPF), also named mitosin, is a large human protein of 3113 amino acid residues. Its expression and localization are cell cycle-dependent. The protein levels are low in G1 phase but elevated from S to early M phase. CENPF is a n...  Read full blog post.

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Gene Symbol CENPF