CENPF Antibody [DyLight 550]

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Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 550

CENPF Antibody [DyLight 550] Summary

Immunogen
A bacterial fusion protein from the C-terminus of human CENPF. [UniProt# P49454]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CENPF
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
This CENPF antibody is useful for Flow Cytometry, Immunoprecipitation, Immunofluorescence/Immunocytochemistry, and Western Blot where a band can be seen at ~330 kDa. In ICC/IF, nuclear staining was observed in HeLa cells.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CENPF Antibody [DyLight 550]

  • AH antigen
  • cell-cycle-dependent 350K nuclear protein
  • CENF
  • CENP-F kinetochore protein
  • CENP-F
  • centromere protein F (350/400kD, mitosin)
  • centromere protein F
  • centromere protein F, 350/400ka (mitosin)
  • centromere protein F, 350/400kDa (mitosin)
  • hcp-1
  • Kinetochore protein CENPF
  • mitosin
  • PRO1779

Background

The kinetophore is a macromolecular structure that is associated with the centromeres of chromosomes and is responsible for establishing and maintaining the connections with the microtubules of the mitotic spindle. The proteins that reside within the chromosomal domain fall into two broad classes: those that associate with the centromere throughout the cell cycle and those that are found at the centromere in a cell cycle specific manner. Members of the first class include CENP-A, CENP-B, and CENP-C. The members of the second class include several ubiquitous cellular proteins such as calmodulin, tubulin, dynein and p34 cdc2 and also CENP-E and CENP-F. CENP-F appears to be the earliest member to interact with the centromere-kinetochore complex. CENP-F also shows a cell-cycle distribution that is both temporally regulated and diverse in terms of the structural components it localizes to and therefore may play a role in several mitotic events. (1-3)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CENPF Antibody (NB500-101R) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB500-101B
DyLight 488 Labeled NB500-101G
DyLight 650 Labeled NB500-101C
HRP Labeled NB500-101H

Additional CENPF Products

CENPF NB500-101R

Bioinformatics Tool for CENPF Antibody (NB500-101R)

Discover related pathways, diseases and genes to CENPF Antibody (NB500-101R). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CENPF Antibody (NB500-101R)

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Pathways for CENPF Antibody (NB500-101R)

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PTMs for CENPF Antibody (NB500-101R)

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Research Areas for CENPF Antibody (NB500-101R)

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Blogs on CENPF.

CENPF: At the Center-o'-mere Mitotic Division (Infographic)
Centromere protein F (CENPF) also known as Mitosin, AH antigen, and kinetochore protein CENPF, is a protein that associates with the centromere-kinetochore complex. CENPF forms both a homodimer and a heterodimer. CENPF can be found in different cellul...  Read full blog post.

CENPF Antibodies as Potential Cancer Markers
Centromere protein F (CENPF), also named mitosin, is a large human protein of 3113 amino acid residues. Its expression and localization are cell cycle-dependent. The protein levels are low in G1 phase but elevated from S to early M phase. CENPF is a n...  Read full blog post.

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Bioinformatics

Gene Symbol CENPF