CDP/CUTL1 Antibody - BSA Free

Western Blot: CDP/CUTL1 Antibody [NBP2-16039] - IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of CUTL1 protein by 2 ug of CUTL1 antibody 7.5% SDS-PAGE The immunoprecipitated CUTL1 protein was detected by CUTL1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Immunocytochemistry/Immunofluorescence: CDP/CUTL1 Antibody [NBP2-16039] - CDP/CUTL1 antibody detects CDP/CUTL1 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDP/CUTL1 stained by CDP/CUTL1 antibody diluted at 1:500. Red: Tau, stained by Tau antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: CDP/CUTL1 Antibody [NBP2-16039] - Sample (30 ug of whole cell lysate) A: Raji B: K562 5% SDS PAGE gel, diluted at 1:500.

Immunocytochemistry/Immunofluorescence: CDP/CUTL1 Antibody [NBP2-16039] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CUTL1 protein stained by CUTL1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CDP/CUTL1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CDP/CUTL1 Antibody - BSA Free (NBP2-16039) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CDP/CUTL1. The exact sequence is proprietary.
• Localization: Golgi apparatus membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CUX1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 164 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CDP/CUTL1 Antibody - BSA Free

• CASP
• CCAAT displacement protein
• CDP
• CDP/Cux
• CDPCCAAT displacement protein
• COY1
• cut (Drosophila)-like 1 (CCAAT displacement protein)
• CUT
• CUTL1
• cut-like 1, CCAAT displacement protein (Drosophila)
• cut-like homeobox 1
• CUX
• CUX1
• FLJ31745
• golgi integral membrane protein 6
• GOLIM6
• homeobox protein cut-like 1
• Homeobox protein cux-1
• Nbla10317
• p100
• p110
• p200
• p75
• protein CASP
• putative protein product of Nbla10317

Background

The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CUX1