| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4459-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81841-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21697-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57705-25ul | |||
![Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody (M01), clone 3B11.Lane 1: CDC25C transfected lysate(53.312 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3B11-Western-Blot-H00000995-M01-img0012.jpg "Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody (M01), clone 3B11.Lane 1: CDC25C transfected lysate(53.312 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 6 ug/ml.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3B11-Immunohistochemistry-Paraffin-H00000995-M01-img0007.jpg)
![Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi ( Cat # H00000995-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody (M01), clone 3B11 (Cat # H00000995-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3B11-Western-Blot-H00000995-M01-img0009.jpg)
![Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - CDC25C monoclonal antibody (M01), clone 3B11 Analysis of CDC25C expression in HeLa.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3B11-Western-Blot-H00000995-M01-img0011.jpg)
![Immunoprecipitation: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C transfected lysate using anti-CDC25C monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CDC25C MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3B11-Immunoprecipitation-H00000995-M01-img0008.jpg)
![Sandwich ELISA: CDC25C Antibody (3B11) [H00000995-M01] - Detection limit for recombinant GST tagged CDC25C is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3B11-Sandwich-ELISA-H00000995-M01-img0010.jpg)
![Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-27820241631628.jpg "Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32173) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29820241420958.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunocytochemistry analysis of HeLa cells labeling CDC25C with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-2920241023428.jpg)
![Flow Cytometry: CDC25C Antibody (JE59-53) [NBP3-32173] - Flow cytometric analysis of HeLa cells labeling CDC25C. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32173, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29202416453230.jpg)
![Western Blot: CDC25C Antibody [NBP2-15827] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/CDC25C-Antibody-Western-Blot-NBP2-15827-img0004.jpg "Western Blot: CDC25C Antibody [NBP2-15827] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody")
![Immunocytochemistry/Immunofluorescence: Cdc25C Antibody [NBP2-15827] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using CDC25C antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Cdc25C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15827-img0003.jpg)
![Immunohistochemistry-Paraffin: Cdc25C Antibody [NBP2-15827] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Cdc25C-Antibody-Immunohistochemistry-Paraffin-NBP2-15827-img0002.jpg)
![Western Blot: Cdc25C Antibody [NBP2-15827] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/Cdc25C-Antibody-Western-Blot-NBP2-15827-img0001.jpg)
![Western Blot: CDC25C Antibody (3E6) [NBP3-15178] - Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate. All lanes: CDC25C antibody at 1.65ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 52, 49, 46 KDa. Observed band size: 60 KDa.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Western-Blot-NBP3-15178-img0004.jpg "Western Blot: CDC25C Antibody (3E6) [NBP3-15178] - Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate. All lanes: CDC25C antibody at 1.65ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 52, 49, 46 KDa. Observed band size: 60 KDa.")
![Immunocytochemistry/Immunofluorescence: CDC25C Antibody (3E6) [NBP3-15178] - Immunofluorescence staining of Hela cells with at CDC25C antibody 1:55, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunocytochemistry-Immunofluorescence-NBP3-15178-img0001.jpg)
![Immunohistochemistry: CDC25C Antibody (3E6) [NBP3-15178] - IHC image of CDC25C antibody diluted at 1:165 and staining in paraffin-embedded human glioma cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunohistochemistry-NBP3-15178-img0003.jpg)
![Immunoprecipitation: CDC25C Antibody (3E6) [NBP3-15178] - Immunoprecipitating CDC25C in HEK293 whole cell lysate. Lane 1: Rabbit control IgG instead of CDC25C antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: CDC25C antibody (3ug) + HEK293 whole cell lysate (500ug). Lane 3: HEK293 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunoprecipitation-NBP3-15178-img0002.jpg)
![Western Blot: CDC25C [p Ser216] Antibody [NB100-81841] - Analysis of extracts from 293 cells untreated or treated with serum using CDC25C [p Ser216] Antibody.](http://images.novusbio.com/fullsize/CDC25C-[p-Ser216]-Antibody-Western-Blot-NB100-81841-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: CDC25C [p Ser216] Antibody [NB100-81841] - Analysis of extracts from 293 cells untreated or treated with serum using CDC25C [p Ser216] Antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CDC25C [p Ser216] Antibody [NB100-81841] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-81841 (left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/CDC25C-[p-Ser216]-Antibody-Immunohistochemistry-Paraffin-NB100-81841-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NB100-81841 [NB100-81841] - Staining of methanol-fixed Hela cells using NB100-81841.](http://images.novusbio.com/fullsize/nb100-81841_rabbit-polyclonal-cdc25c-p-ser216-antibody-11320251555915.jpg)
![Western Blot: CDC25C Antibody (6R0I6) [NBP3-15437] - Western blot analysis of extracts of various cell lines, using CDC25C antibody (NBP3-15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CDC25C-Antibody-6R0I6-Western-Blot-NBP3-15437-img0002.jpg "Western Blot: CDC25C Antibody (6R0I6) [NBP3-15437] - Western blot analysis of extracts of various cell lines, using CDC25C antibody (NBP3-15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (6R0I6) [NBP3-15437] - Immunofluorescence analysis of HeLa cells using CDC25C Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15437_rabbit-cdc25c-mab-6r0i6-72202512435382.jpg)
![Western Blot: CDC25C Antibody (SR2051) [NBP3-21697] - Western blot analysis using NBP3-21697 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21697_rabbit-cdc25c-mab-sr2051-57202317161892.jpg "Western Blot: CDC25C Antibody (SR2051) [NBP3-21697] - Western blot analysis using NBP3-21697 in K562 cell lysate.")
![SDS-Page: Recombinant Human CDC25C Protein [H00000995-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00000995-P01-1.jpg "SDS-Page: Recombinant Human CDC25C Protein [H00000995-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: CDC25C Antibody [NBP2-57705] - Staining of human cell line U-2 OS shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/CDC25C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57705-img0001.jpg "Immunocytochemistry/Immunofluorescence: CDC25C Antibody [NBP2-57705] - Staining of human cell line U-2 OS shows localization to nuclear speckles.")
![Western Blot: CDC25C [p Ser216] Antibody [NBP3-12879] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C (phospho Ser216) antibody (NBP3-12879) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDC25C-[p-Ser216]-Antibody-Western-Blot-NBP3-12879-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: CDC25C [p Ser216] Antibody [NBP3-12879] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C (phospho Ser216) antibody (NBP3-12879) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")