CDC25C Antibody (461908) [Alexa Fluor® 700]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 461908
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 700

CDC25C Antibody (461908) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human CDC25C
  Ser2-Pro473
  Accession # P30307
• Specificity: Detects human, mouse and rat CDC25C in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CDC25A or rhCDC25B is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDC25C Antibody (461908) [Alexa Fluor® 700]

• CDC 25
• CDC25
• CDC25C
• cell division cycle 25 homolog C (S. cerevisiae)
• cell division cycle 25 homolog C (S. pombe)
• cell division cycle 25C
• Dual specificity phosphatase Cdc25C
• EC 3.1.3.48
• mitosis inducer CDC25
• M-phase inducer phosphatase 3
• phosphotyrosine phosphatase

Background

Cell Division Cycle 25C (CDC25C) is a dual serine/threonine and tyrosine phosphatase. There are four splice variants with molecular weights of 46, 47, 49, and 53 kDa. The smaller variants lack key regulatory sites and their upregulation has been observed in prostate cancer. CDC25C regulates the G2/M cell cycle transition by dephosphorylating proteins such as Chk1. Its cellular distribution is controlled by phosphorylations that enhance 14-3-3 binding, block nuclear localization, and inhibit enzymatic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.