CDC25A Antibody

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Western Blot: CDC25A Antibody [NB100-213] - Detection of Human CDC25a by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used ...read more
Western Blot: CDC25A Antibody [NB100-213] - Nuclear extract (50 ug) from HeLa and 293T cells. Antibody:Affinity purified rabbit anti-CDC25a antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure ...read more
Western Blot: CDC25A Antibody [NB100-213] - Nuclear extract (1 mg) from HeLa cells: Antibody used at 2 ug/mg lysate for IP and 1 ug/ml for Western Blot.
Western Blot: CDC25A Antibody [NB100-213] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-CDC25a antibody (lot was used at 1 ug/ml. ...read more
Immunoprecipitation: CDC25A Antibody [NB100-213] - Detection of human CDC25a, using NB100-213, by Immunoprecipitation followed by Western Blot. Sample: Nuclear extract from HeLa cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

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CDC25A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 175 and 225 of human Cell Division Cycle 25A using the numbering given in entry NP_001780.2 (GeneID 993).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDC25A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunoprecipitation 2-10 ug/mg of lysate
Publications
Read Publication using NB100-213.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDC25A Antibody

  • CDC25A
  • CDC25A2
  • CDC25A2-CAG isoform
  • cell division cycle 25 homolog A (S. cerevisiae)
  • cell division cycle 25 homolog A (S. pombe)
  • cell division cycle 25A
  • Dual specificity phosphatase Cdc25A
  • EC 3.1.3.48
  • M-phase inducer phosphatase 1

Background

Cyclin-dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin-dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S-phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDC25A Antibody (NB100-213)(1)

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Bioinformatics

Gene Symbol CDC25A
Entrez