HAF007). Specific bands were detected for CDC25A at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line, CTLL-2 mouse cytotoxic T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC25A Monoclonal Antibody (Catalog # MAB1648) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CDC25A at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1648-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11882-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17816-25ul | |||
![Western Blot: CDC25A Antibody [NB100-213] - Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDC25A-Antibody-Western-Blot-NB100-213-img0009.jpg "Western Blot: CDC25A Antibody [NB100-213] - Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Western Blot: CDC25A Antibody [NB100-213] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-CDC25a antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDC25A-Antibody-Western-Blot-NB100-213-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CDC25A Antibody [NB100-213] - Detection of Human CDC25a by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDC25A-Antibody-Western-Blot-NB100-213-img0008.jpg)
![Western Blot: Rabbit Polyclonal CDC25A Antibody [NB100-213] - Whole cell lysates from MDA-MB-231, SUM159, MCF-7 and HEK293T cells were loaded with 50 ug/lane. 10% SDS-PAGE. CDC25A Antibody (NB100-213) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-213_rabbit-polyclonal-cdc25a-antibody-western-blot-3112024151137..png)
![Proximity Ligation Assay: CDC25A Antibody (3D5) [H00000993-M01] - Analysis of protein-protein interactions between CCNB1 and CDC25A. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-CDC25A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CDC25A-Antibody-3D5-Proximity-Ligation-Assay-H00000993-M01-img0002.jpg "Proximity Ligation Assay: CDC25A Antibody (3D5) [H00000993-M01] - Analysis of protein-protein interactions between CCNB1 and CDC25A. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-CDC25A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Sandwich ELISA: CDC25A Antibody (3D5) [H00000993-M01] - Detection limit for recombinant GST tagged CDC25A is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDC25A-Antibody-3D5-Sandwich-ELISA-H00000993-M01-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CDC25A Antibody [NBP3-17816] - Staining of human cell line HAP1 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CDC25A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17816-img0002.jpg "Immunocytochemistry/Immunofluorescence: CDC25A Antibody [NBP3-17816] - Staining of human cell line HAP1 shows localization to nucleoplasm.")
![SDS-Page: Recombinant Human CDC25A Protein [NBP1-51019] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-CDC25A-Protein-SDS-Page-NBP1-51019-img0001.jpg "SDS-Page: Recombinant Human CDC25A Protein [NBP1-51019] - 15 % SDS-PAGE (3ug)")
![ELISA: Human CDC25A - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31208] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31208_human-cdc25a-ready-to-use-elisa-kit-colorimetric-206202415351080.png "ELISA: Human CDC25A - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31208] - Standard Curve Reference")
![ELISA: Human CDC25A ELISA Kit (Colorimetric) [NBP3-27359] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-27359_human-cdc25a-elisa-kit-colorimetric-114202410295948.png "ELISA: Human CDC25A ELISA Kit (Colorimetric) [NBP3-27359] - Standard Curve Reference")
