CDC25A Antibody (336445) [Alexa Fluor™ Plus 680]

• Reactivity: Hu, Mu, Rt
• Clone: 336445
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

CDC25A Antibody (336445) [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects human, mouse, and rat CDC25A in direct ELISAs and Western blots. In direct ELISA and Western blots, this antibody does not cross-react with recombinant human (rh) CDC25B or rhCDC25C.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDC25A Antibody (336445) [Alexa Fluor™ Plus 680]

• CDC25A
• CDC25A2
• CDC25A2-CAG isoform
• cell division cycle 25 homolog A (S. cerevisiae)
• cell division cycle 25 homolog A (S. pombe)
• cell division cycle 25A
• Dual specificity phosphatase Cdc25A
• EC 3.1.3.48
• M-phase inducer phosphatase 1

Background

Cell Division Cycle 25A (CDC25A) phosphatase removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). Overexpression of CDC25A in small mammary carcinomas has been associated with a poor patient survival prognosis (2). Levels of CDC25A activity are highest in S phase of the cell cycle, where it is suspected to be involved in “checkpoint” control of cell cycle progression (3). Induction of DNA damage with ultraviolet or ionizing radiation causes an increase in CDC25A ubiquinylation and proteosomal degradation, leading to cell cycle block between G1 and S phases (4). One of its major substrates, the kinase CDC2, is activated by dephosphorylation (5). Recombinant CDC25A is truncated to remove the N-terminal regulatory domains and is fully active.

1. Draetta, G. and J. Eckstein (1997) Biochim. Biophys. Acta. 1332:M53.
2. Cangi, M. et al. (2000) J. Clin. Invest. 106:753.
3. Hoffmann, I. et al. (1995) EMBO J. 13:4302.
4. Sagata, N. (2002) Science 298:1905.
5. Gautier, J. et al. (1991) Cell 67:197.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.