CDC25A Antibody (336445) [CoraFluor™ 1]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 336445
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

CDC25A Antibody (336445) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human CDC25A
  Glu2-Leu523
  Accession # AAA58415
• Specificity: Detects human, mouse, and rat CDC25A in direct ELISAs and Western blots. In direct ELISA and Western blots, this antibody does not cross-react with recombinant human (rh) CDC25B or rhCDC25C.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDC25A
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for CDC25A Antibody (336445) [CoraFluor™ 1]

• CDC25A
• CDC25A2
• CDC25A2-CAG isoform
• cell division cycle 25 homolog A (S. cerevisiae)
• cell division cycle 25 homolog A (S. pombe)
• cell division cycle 25A
• Dual specificity phosphatase Cdc25A
• EC 3.1.3.48
• M-phase inducer phosphatase 1

Background

The activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S phase and G2 for CDC25B. CDC25C is mainly expressed in G2 phase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC25A